Product Name:
EphA3-pY779
Product Number:
ab-pk608
Target Full Name: Ephrin type-A receptor 3 protein-tyrosine kinase
Target Alias: EPA3; Eph receptor A3; Ephrin type-A receptor 3; ETK; ETK1; HEK; HEK4; Kinase EphA3; Tyrosine-protein kinase receptor REK4; TYRO4; CCDS2922.1; ENSG00000044524
Product Type Specific: EphA3 protein kinase phosphosite-specific antibody
Antibody Code: PK608
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y779
Protein UniProt: P29320
Protein SigNET: EphA3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human EphA3 sequence peptide Cat. No.: PE-04AGN65
Antibody Immunogen Sequence: EAA(pY)TTR(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues E776 to R782. Y779 phosphorylation stimulates phosphotransferase activity. Phosphorylation regulates cell adhesion, cytoskeletal reorganization, and induces molecular association with CRK. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is the major in vivo phosphorylation site in EphA3 (≥723 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.4 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human | Rhesus Macaque | Rat | Mouse | Chicken | Frog | Zebra fish
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 120-130 kDa.
Antibody Cross Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Rat | Mouse | Chicken | Frog | Zebra fish.
Related Product 1: EphA3-pY779 blocking peptide
Related Product 2: EphA1 Y599 phosphorylation antibody (Cat. No.: AB-PK928)
Related Product 3: EphA1 Y781 phosphorylation antibody (Cat. No.: AB-PK605)
Related Product 4: EphA2 Y588 phosphorylation antibody (Cat. No.: AB-PK606)
Related Product 5: EphA2 Y772 phosphorylation antibody (Cat. No.: AB-PK607)
Related Product 6: EphA4 Y596 phosphorylation antibody (Cat. No.: AB-PK929)
Related Product 7: EphA4 Y602 phosphorylation antibody (Cat. No.: AB-PK930)
Related Product 8: EphA4 Y779 phosphorylation antibody (Cat. No.: AB-PK931)
Related Product 9: EphB1 Y594 phosphorylation antibody (Cat. No.: AB-PK609)
Related Product 10: EphB2 expression antibody (Cat. No.: AB-NK267-1)
Scientific Background: EphA3 is a receptor protein-tyrosine kinase of the TK group and Eph family. It features an extracellular domain (with Cys-rich region and 2 fibronectin type III repeats), a single transmembrane domain, and a cytoplasmic protein-tyrosine kinase domain. It is activated by binding ephrin-A1, A2, A3, A4 or A5 (with high affinity for EFNA5), and subsequent receptor dimerization. It mediates contact-dependent cell signalling, primarily in nervous system development and tissue patterning. It regulates cell migration and adhesion by binding membrane-bound ephrin-A ligands, often promoting cell repulsion. Phosphorylation of Tyr-596, Tyr-602 and Tyr-779 increases phosphotransferase activity and interaction with Crk. It is widely expressed in human tissues. Upregulation of EphA3 is common in glioblastomas, lung cancer and melanomas. Its mutation pattern in human tumours is very characteristic of a tumour suppressor protein. Somatic mutations in EPHA3 are found in breast, lung, and pancreatic cancers, often leading to reduced receptor function. EphA3 function is inhibited and EFNA5 binding interrupted with the V133E, and F152L mutations. EphA3 is linked to Pleomorphic Rhabdomyosarcoma, which is a rare disease which is related to colorectal cancer, and can also affect the uterus, heart, and skeletal muscle tissues. This description may include information annotated by UniProt and/or Google AI.