Product Name:
EphA2-pY588
Product Number:
ab-pk606
Target Full Name: Ephrin type-A receptor 2 protein-tyrosine kinase
Target Alias: ECK; Eph receptor A2; Epithelial cell kinase; Kinase EphA2; MPK-5; SEK2; SEK-2; Tyrosine-protein kinase receptor ECK; RCC2; CCDS169.1; ENSG00000142627
Product Type Specific: EphA2 protein kinase phosphosite-specific antibody
Antibody Code: PK606
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y588
Protein UniProt: P29317
Protein SigNET: EphA2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human EphA2 sequence peptide Cat. No.: PE-04ALW99
Antibody Immunogen Sequence: LKT(pY)VDP(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues L585 to P591; in the EphA2-TM domain located prior to the kinase catalytic domain. One of the major in vivo sites of phosphorylation in EphA2.Y588 phosphorylation stimulates phosphotransferase activity and regulates cell adhesion, cell differentiation, cell motility and molecular association, including inducing interaction with Nck1, VAV2 and VAV3. One of the major in vivo sites of phosphorylation in EphA2 (≥655 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). EphA2 is known to be phosphorylated at this site in vitro by EphA2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with immunogen peptide on dot blots.
Antibody Species Reactivity: Human | Chimpanzee | Rhesus Macaque | Mouse | Platypus | Chicken | Frog
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 115-130 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus Macaque | Mouse | Platypus | Chicken | Frog.
Related Product 1: EphA2-pY588 blocking peptide
Related Product 2: EphA1 Y599 phosphorylation antibody (Cat. No.: AB-PK928)
Related Product 3: EphA1 Y781 phosphorylation antibody (Cat. No.: AB-PK605)
Related Product 4: EphA2 Y772 phosphorylation antibody (Cat. No.: AB-PK607)
Related Product 5: EphA3 Y779 phosphorylation antibody (Cat. No.: AB-PK608)
Related Product 6: EphA4 Y596 phosphorylation antibody (Cat. No.: AB-PK929)
Related Product 7: EphA4 Y602 phosphorylation antibody (Cat. No.: AB-PK930)
Related Product 8: EphA4 Y779 phosphorylation antibody (Cat. No.: AB-PK931)
Related Product 9: EphB1 Y594 phosphorylation antibody (Cat. No.: AB-PK609)
Related Product 10: EphB2 expression antibody (Cat. No.: AB-NK267-1)
Scientific Background: EphA2 is a 130 kDa protein-tyrosine kinase of the TK group and Eph family. It is a receptor that displays promiscuous binding to membrane-bound ligands of the ephrin-A family on adjacent cells. It is activated by binding ephrin-A1, A2, A3, A4 or A5, and subsequent dimerization. It features a single cytoplasmic kinase domain and an extracellular region with a Cys-rich domain and two fibronectin type III repeats. Phosphorylation of Tyr-735 increases phosphotranserase activity and interaction with PIK3R1. EphA2 activation regulates integrin-dependent adhesion, cellular migration, proliferation, and differentiation. It is present at low levels in normal adult epithelial cells, and primarily found on the plasma membrane and at cell junctions. EphA2 also participates in bone remodeling by directly regulating both osteoclastogenesis and osteoblastogenesis. EphA2 may be an oncoprotein (OP). Significantly elevated EphA2 expression has been observed in many types of human cancer and appears to be involved in mediating communication between RAS-PI3K/Akt and RAS-MAPK intracellular signalling pathways, which are both known to promote tumorigenesis. EphA2 is required for UV-radiation induced apoptosis. UV-radiation is a potent carcinogen responsible for the development of melanoma. However, further characterization of melanoma cell lines revealed either a pro-apoptotic or anti-apoptotic role for EphA2 dependent upon the cellular context. Additionally, studies performed in breast cancer cell lines revealed a link between EphA2 expression levels and changes in cytoskeletal morphology and the recruitment of disintegrin and metalloprotease-10 (MMP10) enzymes, which also implicates a role for theEphA2 protein in the promotion of tumour invasion and metastasis. It also acts as a receptor for KSHV and Hepatitis C virus entry into host cells. This description may include information annotated by UniProt and/or Google AI.