Product Name:
EphA1-pY781
Product Number:
ab-pk605
Target Full Name: Ephrin type-A receptor 1 protein-tyrosine kinase
Target Alias: EPA1; Eph; Eph receptor A1; Ephrin type-A receptor 1; EphT; EphT1; MGC163163; Tyrosine-protein kinase receptor Eph; CCDS5884.1; P21709; A1L3V3; ENSG00000146904
Product Type Specific: EphA1 protein kinase phosphosite-specific antibody
Antibody Code: PK605
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y781
Protein UniProt: P21709
Protein SigNET: EphA1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human EphA1 sequence peptide Cat. No.: PE-04ALV80
Antibody Immunogen Sequence: DGT(pY)ETQ(bA)C
Antibody Immunogen Description: Corresponds to amino acid residues D778 to Q784. Y781 phosphorylation stimulates phosphotransferase activity. This phosphosite is located in the kinase activation loop between catalytic subdomains VII and VIII. This is the major in vivo phosphorylation site in EphA1 (≥255 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.This antibody was also subject to negative purification over phosphotyrosine-agarose.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Medium-strong immunoreactivity of a target-sized protein by Western blotting in HeLa cells.
Antibody Species Reactivity: Human | Rhesus Macaque | Dog | Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 95-105 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus Macaque | Dog | Mouse.
Related Product 1: EphA1-pY781 blocking peptide
Related Product 2: EphA1 Y599 phosphorylation antibody (Cat. No.: AB-PK928)
Related Product 3: EphA2 Y588 phosphorylation antibody (Cat. No.: AB-PK606)
Related Product 4: EphA2 Y772 phosphorylation antibody (Cat. No.: AB-PK607)
Related Product 5: EphA3 Y779 phosphorylation antibody (Cat. No.: AB-PK608)
Related Product 6: EphA4 Y596 phosphorylation antibody (Cat. No.: AB-PK929)
Related Product 7: EphA4 Y602 phosphorylation antibody (Cat. No.: AB-PK930)
Related Product 8: EphA4 Y779 phosphorylation antibody (Cat. No.: AB-PK931)
Related Product 9: EphB1 Y594 phosphorylation antibody (Cat. No.: AB-PK609)
Related Product 10: EphB2 expression antibody (Cat. No.: AB-NK267-1)
Scientific Background: EphA1 is a 108-130 kDa protein-tyrosine kinase of the TK group and Eph family. It is activated by binding of ephrin-A1 (EFNA1)on a presenting cell, which is likely to induce dimerization and autophosphorylation. This kinase is moderate to highly expressed in most tested human tissues, and located in the plasma membrane, cytoplasm, and sometimes in the nucleus. Orthologues of EphA1 are highly conserved in mammals and birds. EphA1 has been implicated in mediating embryogenesis and early developmental, particularly in the nervous system. It regulates angiogenesis, cell attachment, and inhibits motility. EphA1 seems to be a marker of the differentiated normal epidermis, and its downregulation in nonmelanoma skin cancer may contribute to carcinogenesis of these very frequent human tumours. EphA1 represents a new potential prognostic marker and therapeutic target in nonmelanoma skin cancer. EphA1 has also been linked with the development of glioblastomas, head and neck cancer and breast carcinomas (pleomorphic lobular). This description may include information annotated by UniProt and/or Google AI.