Product Name:
Cav1.3
Product Number:
ab-nn226-1
Target Full Name: Voltage-dependent L-type calcium channel subunit alpha-1D
Target Alias: alpha-1 polypeptide; CAC1D_HUMAN; CACH3; CACN4; CACNA 1D; Cacna1d; CACNL1A2; Calcium channel; Calcium channel L type alpha 1 polypeptide isoform 2; Calcium channel neuroendocrine/brain type alpha 1 subunit; Calcium channel voltage dependent L type alpha 1D subunit; CCHL1A2; isoform 2; L type; Voltage dependent L type calcium channel subunit alpha 1D; Voltage gated calcium channel alpha 1 subunit; Voltage gated calcium channel alpha subunit Cav1.3; Voltage gated calcium channel subunit alpha Cav1.3; Voltage-gated calcium channel subunit alpha Cav1.3
Product Type Specific: Caclium channel pan-specific antibody
Antibody Code: NN226-1
Antibody Target Type: Pan-specific
Protein UniProt: Q01668
Protein SigNET: Cav1.3
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a Kappa
Antibody Immunogen Source: Fusion protein with peptide sequence of rat Cav1.3 (Uniprot ID P27732)
Antibody Immunogen Sequence: DNKVTIDDYQEEAEDKD
Antibody Immunogen Description: Corresponds to amino acid residues D859-D875.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~250 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse
Antibody Positive Control: 1 µg/ml of SMC-301 was sufficient for detection of Cav1.3 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Cross Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse.
Related Product 1: Cav beta 1 pan-specific antibody (Cat. No.: AB-NN222-1)
Related Product 2: Cav beta 2 pan-specific antibody (Cat. No.: AB-NN228-1)
Related Product 3: Cav beta 4 pan-specific antibody (Cat. No.: AB-NN223-1)
Related Product 4: Cav1.2 pan-specific antibody (Cat. No.: AB-NN225-1)
Related Product 5: Cav3.1 pan-specific antibody (Cat. No.: AB-NN227-1)
Scientific Background: Cav1.3, also known as the calcium channel, voltage-dependent, L type, alpha 1D subunit is encoded by the CACNA1D gene. The 250 kDa protein facilitates calcium influx into excitable cells, crucial for hearing, cardiac pacemaking, and neuronal firing. It is distinct for opening at more negative voltages compared to other L-type channels, playing a key role in pacemaker activity and synaptic plasticity. Cav1.3 mediates "long-lasting" (L-type) currents that are sensitive to dihydropyridines (DHPs), used to treat channelopathies, though they are generally more sensitive to channel inhibitors compared to other types. The protein consists of four transmembrane domains (I–IV), each with six segments (S1–S6), with S4 acting as a voltage sensor. It acts as the pore-forming a1D subunit, often interacting with regulatory b, g, and a2d subunits. Cav1.3 subunits are primarily expressed in neurons and neuroendocine cells. Cav1.3 is also found in the atria, and may figure prominently in atrial arrhythmias (1). Cav1.3 also carries the primary sensory receptors of the mammalian cochlea, and are also expressed in the electromotile outer hair cells (2). Crucial for pacemaker activity in the sinoatrial node and for regulating neuronal firing in the substantia nigra, with roles in anxiety and addiction. Mutations are linked to a syndrome involving congenital deafness and sick sinus syndrome (bradycardia). Gain-of-function mutations are linked to autism spectrum disorders and epilepsy. This description may include information annotated by UniProt and/or Google AI.