Product Name:
Cav beta 2
Product Number:
ab-nn228-1
Target Full Name: Voltage-dependent L-type calcium channel subunit beta-2
Target Alias: Cacnlb2; cacnb2; CAB2
Product Type Specific: Calcium channel pan-specific antibody
Antibody Code: NN228-1
Antibody Target Type: Pan-specific
Protein UniProt: Q08289
Protein SigNET: Cav beta 2
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Synthetic peptide patterned after rat CavBeta2 (Uniprot ID Q8VGC3)
Antibody Immunogen Sequence: KFYSSKSGGNSSSSLG
Antibody Immunogen Description: Corresponds to amino acid residues K189-G205.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~78 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-332 was sufficient for detection of Cavβ2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross reactivity against Cavβ1, Cavβ3, Cavβ4.
Related Product 1: Cav beta 1 pan-specific antibody (Cat. No.: AB-NN222-1)
Related Product 2: Cav beta 4 pan-specific antibody (Cat. No.: AB-NN223-1)
Related Product 3: Cav1.2 pan-specific antibody (Cat. No.: AB-NN225-1)
Related Product 4: Cav1.3 pan-specific antibody (Cat. No.: AB-NN226-1)
Related Product 5: Cav3.1 pan-specific antibody (Cat. No.: AB-NN227-1)
Scientific Background: Cav Beta subunits are involved in the transport of the pore forming alpha1 subunit to the plasma membrane (1). They act as chaperones, increasing the trafficking of the pore-forming a1 subunit (Cav1.2) to the plasma membrane, thus increasing calcium current density.They also shield an ER Retention signal on the alpha1 subunit, thereby guiding the pore-forming subunit to the target membrane (2, 3). They also determine the biophysical properties of the calcium channel (3). The Cavb2 protein (encoded by the CACNB2 gene) is a cytosolic auxiliary subunit that plays an essential, regulatory role in high-voltage activated (HVA) L-type calcium channels (LTCCs), particularly in cardiomyocytes. It is the predominant beta isoform in human and murine hearts. It is critical for cardiac development; global knockout of the Cavb2 gene is lethal at early embryonic stages, leading to a lack of cardiac contractions. A portion of Cavb2 is targeted to the nucleus in cardiomyocytes, where it regulates gene expression and participates in cellular functions independent of LTCC activity. Downregulation of the nuclear fraction of Cavb2 promotes cardiomyocyte hypertrophy by reducing calpastatin expression and activating calpain-dependent signaling pathways. It is a member of the MAGUK (membrane-associated guanylate kinase) protein family and contains a conserved modular core structure flanked by variable regions. The conserved core features SH3 (Src homology 3) and GK (guanylate kinase) domains, connected by a HOOK region. Mutations in the CACNB2 gene are associated with cardiac diseases like Brugada syndrome and sudden death syndromes. This description may include information annotated by UniProt and/or Google AI.