Product Name:
Cav beta 1
Product Number:
ab-nn222-1
Target Full Name: Voltage-dependent L-type calcium channel subunit beta-1
Target Alias: CACNB1; CAB1; CACNLB1; voltage dependent L type calcium channel beta 1 subunit
Product Type Specific: CACB1 calcium channel pan-specific antibody
Antibody Code: NN222-1
Antibody Target Type: Pan-specific
Protein UniProt: Q02641
Protein SigNET: Cav beta 1
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Synthetic peptide patterned after rat CavB1 (Uniprot ID P54283)
Antibody Immunogen Sequence: PMEVFDPSPQGKYSKR
Antibody Immunogen Description: Corresponds to amino acid residues P19-R34.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium potency. Detects ~80 and 55 kDa proteins in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-317 was sufficient for detection of Cavβ1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactivity against Cav Beta4.
Related Product 1: Cav beta 2 pan-specific antibody (Cat. No.: AB-NN228-1)
Related Product 2: Cav beta 4 pan-specific antibody (Cat. No.: AB-NN223-1)
Related Product 3: Cav1.2 pan-specific antibody (Cat. No.: AB-NN225-1)
Related Product 4: Cav1.3 pan-specific antibody (Cat. No.: AB-NN226-1)
Related Product 5: Cav3.1 pan-specific antibody (Cat. No.: AB-NN227-1)
Scientific Background: CACNB1 (Calcium Voltage-Gated Channel Auxiliary Subunit Beta 1) is a critical regulatory protein, specifically the b1 subunit of the L-type calcium channel (dihydropyridine receptor) in skeletal muscle. It binds to the pore-forming a1S subunit to promote membrane targeting, increase calcium current, and modulate voltage activation, playing a key role in excitation-contraction coupling. It plays an important role in the calcium channel by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. CACNB1 is heavily expressed in skeletal muscle and cardiac tissues, and it is primarily localized in the T-tubules and sarcolemma of skeletal muscle cells. It increases the peak calcium current and manages the trafficking of the channel complex to the plasma membrane. Alternative splicing occurs at this locus and three transcript variants encoding three distinct isoforms have been identified (1, 2). Genetic variants in CACNB1, causing a loss of b1 subunits, lead to significantly reduced levels of the a1S subunit, causing muscle dysfunction. This description may include information annotated by UniProt and/or Google AI.