Product Name:
Cav1.2
Product Number:
ab-nn225-1
Target Full Name: Voltage dependent L type calcium channel subunit alpha 1D
Target Alias: CACH3; CACN4; CACNA 1D; CACNL1A2; alpha-1 subunit voltage-dependent calcium channel; calcium channel voltage-dependent L type alpha 1C subunit1; voltage-gated calcium channel alpha subunit Cav1.2; calcium channel L type; alpha 1 polypeptide isoform 1 cardic muscle; calcium channel cardic dihydropyridine-sensitive alpha-1 subunit; voltage-gated L-type calcium channel Cav1.2 alpha 1 subunit splice variant 10; DHPR alpha-1 subunit; Voltage-gated calcium channel subunit alpha Cav1.2; Calcium channel L type alpha-1 polypeptide isoform 1 cardiac muscle
Product Type Specific: Calcium channel pan-specific antibody
Antibody Code: NN225-1
Antibody Target Type: Pan-specific
Protein UniProt: P15381
Protein SigNET: Cav1.2
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with intracellular carboxyl terminus of rabbit Cav1.2 (Uniprot ID G1T5I1)
Antibody Immunogen Description: Corresponds to amino acid residues R1507-T1733.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~240 kDa protein (varies with cell background due to glycosylation).
Antibody Species Reactivity: Human | Rat | Mouse | Hamster
Antibody Positive Control: 1 µg/ml of SMC-300 was sufficient for detection of Cav1.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Medium-High
Related Product 1: Cav beta 1 pan-specific antibody (Cat. No.: AB-NN222-1)
Related Product 2: Cav beta 2 pan-specific antibody (Cat. No.: AB-NN228-1)
Related Product 3: Cav beta 4 pan-specific antibody (Cat. No.: AB-NN223-1)
Related Product 4: Cav1.3 pan-specific antibody (Cat. No.: AB-NN226-1)
Related Product 5: Cav3.1 pan-specific antibody (Cat. No.: AB-NN227-1)
Scientific Background: The Cav1.2 protein is a pore-forming a1 subunit of the L-type voltage-dependent calcium channel, encoded by the CACNA1C gene. It acts as a major calcium entry pathway in excitable cells, and is important for cardiac muscle contraction, smooth muscle contraction, and neuronal gene expression (1). As part of a multi-protein complex, the pore-forming a1c subunit interacts with the auxiliary subunits b, a2d and sometimes g. These regulate trafficking and gating. It is a High-Voltage-Activated (HVA) channel, meaning it opens in response to strong membrane depolarization. It is characterized by long-lasting (L-type) calcium currents. Its activity is regulated by calmodulin (CaM) and phosphorylation, which modulate Ca2+-dependent inactivation (CDI) and facilitation (CDF). It is primarily expressed in cardiac myocytes (predominant channel), smooth muscle, and neurons. Cav1.2 may be associated with a variant of Long QT syndrome called Timothy's syndrome (2, 3) and also with Brugada syndrome. Dysfunction of Cav1.2 is also linked to autism and bipolar disease (3). This description may include information annotated by UniProt and/or Google AI.