Product Name:
GABA-B Receptor 1
Product Number:
ab-nn259-1
Target Full Name: Gamma-aminobutyric acid type B receptor subunit 1
Target Alias: GABA-B receptor 1; GABA-B-R1; GABR1_Human; Gamma aminobutyric acid receptor 1; GB1; GPRC3A
Product Type Specific: GABBR1 G protein-coupled receptor pan-specific antibody
Antibody Code: NN259-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9UBS5
Protein SigNET: GABA B Receptor 1
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with cytoplasmic C-terminus of rat GABA(B)R1 (Uniprot ID Q9Z0U4)
Antibody Immunogen Description: Corresponds to amino acid residues 873-977.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~115 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-403 was sufficient for detection of GABA(B)R1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: No cross-reactivity against GABA(B)R2.
Related Product 1: GABA-A Receptor Alpha2 pan-specific antibody (Cat. No.: AB-NN250-1)
Related Product 2: GABA-A Receptor Alpha4 pan-specific antibody (Cat. No.: AB-NN251-1)
Related Product 3: GABA-A Receptor Alpha5 pan-specific antibody (Cat. No.: AB-NN252-1)
Related Product 4: GABA-A Receptor Beta1 pan-specific antibody (Cat. No.: AB-NN253-1)
Related Product 5: GABA-A Receptor Beta3 pan-specific antibody (Cat. No.: AB-NN254-1)
Related Product 6: GABA-A Receptor Epsilon pan-specific antibody (Cat. No.: AB-NN255-1)
Related Product 7: GABA-B Receptor 2 pan-specific antibody (Cat. No.: AB-NN256-2)
Scientific Background: GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system, and it reduces the ability of nerve cells to send or receive chemical messages, producing a calming effect to counteract anxiety, stress and fear. This small molecule interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptors. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kDa protein and GABA(B)R1b is a 95 kDa protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1). This description may include information annotated by UniProt and/or Google AI.