Product Name:
GABA-A Receptor Alpha4
Product Number:
ab-nn251-1
Target Full Name: Gamma-aminobutyric acid receptor subunit alpha-4
Target Alias: GABA A receptor subunit alpha 4; GABRA4; Gamma aminobutyric acid A receptor Alpha 4
Product Type Specific: GABRA4 chloride channel pan-specific antibody
Antibody Code: NN251-1
Antibody Target Type: Pan-specific
Protein UniProt: P48169
Protein SigNET: GABA-A Receptor Alpha4
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with cytoplasmic C-terminus of rat GABA-A-Receptor Alpha 4 (Uniprot ID P28471)
Antibody Immunogen Sequence: SSKTTAAQESSETTPKAHLASSPNPFSRANAAETISAAARG
Antibody Immunogen Description: Corresponds to amino acid residues S410-G450.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~60 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Rat | Mouse.
Antibody Positive Control: A 1:100 dilution of SMC-489 was sufficient for detection of GABA-A R, Alpha4 in 2 0µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with other GABA-A-Receptor Alpha subunits.
Related Product 1: GABA-A Receptor Alpha2 expression antibody (Cat. No.: AB-NN250-1)
Related Product 2: GABA-A Receptor Alpha5 expression antibody (Cat. No.: AB-NN252-1)
Related Product 3: GABA-A Receptor Beta1 expression antibody (Cat. No.: AB-NN253-1)
Related Product 4: GABA-A Receptor Beta3 expression antibody (Cat. No.: AB-NN254-1)
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Scientific Background: GABRA4 is an alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA). The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). It commonly binds to GABA to regulate brain excitability and is associated with autism, epilepsy, and developmental delays. GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2). GABAARs containing alpha-4 are predominantly extrasynaptic, and contributie to tonic inhibition in dentate granule cells and thalamic relay neurons. GABAAR containing alpha-4-beta-3-delta subunits can simultaneously bind GABA and histamine where histamine binds at the interface of two neighboring beta subunits, which has been implicated in the regulation of sleep and wakefulness. In addition to its role in the brain, GABRA4 is essential for mediating autophagy and managing proinflammatory responses in macrophages, acting as a regulator of innate immunity. Mutations and variants in the GABRA4 gene are linked to epilepsy, developmental delay, autism spectrum disorder, and behavioral abnormalities. This description may include information annotated by UniProt and/or Google AI.