Product Name:
GABA-A Receptor Beta3
Product Number:
ab-nn254-1
Target Full Name: Gamma-aminobutyric acid receptor subunit beta-3
Target Alias: ECA5; GABA alpha receptor beta-2 subunit; GABA(A) receptor subunit beta-3; GABAA receptor beta 3 subunit; GABAA receptor subunit beta 3; GABR B3; Gabrb3; Gamma aminobutyric acid (GABA) A receptor beta 3; Gamma aminobutyric acid receptor subunit beta 3; Gamma-aminobutyric acid receptor subunit beta-3; GBRB3_HUMAN; MGC9051 antibody
Product Type Specific: Chloride channel pan-specific antibody
Antibody Code: NN254-1
Antibody Target Type: Pan-specific
Protein UniProt: P28472
Protein SigNET: P28472
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein amino acids 370-433 of mouse GABA-A-R-Beta3
Antibody Modification: Chloride channel pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~55 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-339 was sufficient for detection of Beta3 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: No cross-reactivity against GABA-A-R-Beta 2 or –Beta1.
Related Product 1: GABA-A Receptor Alpha2 pan-specific antibody (Cat. No.: AB-NN250-1)
Related Product 2: GABA-A Receptor Alpha4 pan-specific antibody (Cat. No.: AB-NN251-1)
Related Product 3: GABA-A Receptor Alpha5 pan-specific antibody (Cat. No.: AB-NN252-1)
Related Product 4: GABA-A Receptor Beta1 pan-specific antibody (Cat. No.: AB-NN253-1)
Related Product 5: GABA-A Receptor Epsilon pan-specific antibody (Cat. No.: AB-NN255-1)
Related Product 6: GABA-B Receptor 1 pan-specific antibody (Cat. No.: AB-NN259-1)
Related Product 7: GABA-B Receptor 2 pan-specific antibody (Cat. No.: AB-NN256-2)
Scientific Background: The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2).