Product Name:
PYKSD8
Product Number:
ab-pg005
Product Type Specific: Generic phosphospecific antibody
Antibody Code: PG005
Antibody Target Type: Generic phosphospecific
Antibody Phosphosite: Generic phospho
Protein SigNET: PYKSD8
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PYKSD8 phosphopeptides that featured the sequence W(pY)RAPE
Antibody Immunogen Description: Protein-serine/threonine kinase catalytic subdomain VII region.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Multicellular eukaryotes.
Related Product 1: PYK generic phosphotyrosine-specific antibody (Cat. No.: AB-PG001)
Related Product 2: PYK-Ultra generic phosphotyrosine-specific antibody (Cat. No.: AB-PG001U)
Related Product 3: Phosphotyrosine pan-specific antibody (Cat. No.: AB-CN005-2)
Scientific Background: This antibody cross-reacts with the phosphorylated form of the tyrosine residue that appears to be an inhibitory site in most protein-serine.threonine kinases (but not protein-tyrosine kinases), which is highly conserved and located two amino acid residues on the N-terminal side of the conserved alanine residue in kinase subdomain VIII "APE" sequence. Mutation of this site with either phosphomimetic glutamate or aspartate residues, or with a phenylalanine residues leads to loss of protein-serine/threonine kinase activity for a wide range of protein kinases. This description may include information annotated by UniProt and/or Google AI.