Product Name:
PYK
Product Number:
ab-pg001
Product Type Specific: Generic phosphotyrosine-specific antibody
Antibody Code: PG001
Antibody Target Type: Generic phosphotyrosine-specific
Antibody Phosphosite: Generic phospho
Protein SigNET: PYK
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Over 200 diverse phosphotyrosine phosphorylation site peptides
Antibody Immunogen Description: Diverse locations.
Production Method: Over 400 immunizing phosphopeptides were produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purities were assessed by analytical hplc and the amino acid sequences confirmed by mass spectrometry analysis. These peptides were coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto agarose columns to which a phosphotyrosine-beta-alanine-cysteine was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -30°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Multicellular eukaryotes.
Related Product 1: PYK-Ultra generic phosphotyrosine-specific antibody (Cat. No.: AB-PG001U)
Related Product 2: Phosphotyrosine pan-specific antibody (Cat. No.: AB-CN005-2)
Related Product 3: PYKSD8 generic phospho-specific antibody (Cat. No.: AB-PG005)