Product Name:
SARS-CoV2_S:333-353
Product Number:
ab-nncov2s-5
Product Type Specific: SARS-CoV-2 virus spike S1 subunit (receptor binding domain) protein pan-specific antibody
Antibody Code: NNCOV2S-5
Antibody Target Type: Pan-specific
Protein UniProt: P0DTC2
Protein SigNET: SARS-CoV2_S:333-353
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human SARS-CoV-2-Spike protein
Antibody Immunogen Sequence: SNFRVQPTESIVRFPNITNLC
Antibody Immunogen Description: Corresponds to amino acid residues S333 to L353.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.65 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: SARS-CoV-2.
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Scientific Background: The SARS-CoV-2 virus spike protein (S) attaches the virion to the cell membrane by interacting with primarily the host receptor angiotensin-converting enzyme-2, initiating the infection. During the infection, the S protein is cleaved into two subunit. This cleavage can be catalyzed by TMPRSS2 or during endocytosis by host CSTL. When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane. When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane. Alternatively, S can use the NRP1/NRP2, integrin and ASGR1 as entry receptors. The use of NRP1/NRP2 receptors by the S protein for host cell entry may explain the infection of human olfactory epithelial cells, which express these proteins at high levels but ACE2 at low levels. This description may include information annotated by UniProt and/or Google AI.