Product Name:
SARSCoV2-N-156-170
Product Number:
ab-nncov2n-1
Product Type Specific: SARS-CoV-2 virus nucleocapsid protein pan-specific antibody
Antibody Code: NNCOV2N-1
Antibody Target Type: Pan-specific
Protein UniProt: P0DTC9
Protein SigNET: SARSCoV2-N-156-170
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human SARSCoV2_Nucleocapsid protein
Antibody Immunogen Sequence: AIVLQLPQGTTLPKGC
Antibody Immunogen Description: Corresponds to amino acid residues A156 to G170.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.4 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: SARS-CoV-2.
Related Product 1: SARS-CoV2_1a: NSP1 -151-168 expression antibody (Cat. No.: AB-NNCOV2-1A-1)
Related Product 2: SARS-CoV2_1a: NSP3 -876-890 expression antibody (Cat. No.: AB-NNCOV2-1A-3)
Related Product 3: SARS-CoV2_1a:NSP-2 -735-750 expression antibody (Cat. No.: AB-NNCOV2-1A-2)
Related Product 4: SARS-CoV2_1b: NSP13 - 6713-6735 expression antibody (Cat. No.: AB-NNCOV2-1B-3)
Related Product 5: SARS-CoV2_1b: NSP14 -5606-5619 expression antibody (Cat. No.: AB-NNCOV2-1B-1)
Related Product 6: SARS-CoV2_1b: NSP15 -6053-6071 expression antibody (Cat. No.: AB-NNCOV2-1B-2)
Related Product 7: SARS-CoV2_M:3-23 expression antibody (Cat. No.: AB-NNCOV2M-1)
Related Product 8: SARS-CoV2_S:333-353 expression antibody (Cat. No.: AB-NNCOV2S-5)
Related Product 9: SARS-CoV2_S:450-467 expression antibody (Cat. No.: AB-NNCOV2S-6)
Related Product 10: SARS-CoV2_S:480-494 expression antibody (Cat. No.: AB-NNCOV2S-7)
Scientific Background: The SARS-CoV-2 virus nucleocapsid (N) protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral RNA genome and membrane protein M. It plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. It attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions. It may block host chemokine function in vivo, facilitating viral replication and transmission.It acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG). It acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury. It disrupts the association betwen NLRP3 and ABHD8, enhancing NLRP3 stability and leading to increased inflammasome activation. This description may include information annotated by UniProt and/or Google AI.