Product Name:
SARS-CoV2_M:3-23
Product Number:
ab-nncov2m-1
Product Type Specific: SARS-CoV-2 virus membrane protein pan-specific antibody
Antibody Code: NNCOV2M-1
Antibody Target Type: Pan-specific
Protein UniProt: P0DTC5
Protein SigNET: SARS-CoV2_M:3-23
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human SARS-CoV2_Membrane protein
Antibody Immunogen Sequence: CADSNGTITVEELKKLLEQWN
Antibody Immunogen Description: Corresponds to amino acid residues A3 to N23.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: SARS-CoV-2.
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Scientific Background: The SARS-CoV-2 virus membrane (M) protein is a component of the viral envelope that plays a central role in virus morphogenesis and assembly via its interactions with other viral proteins. The M protein forms a mushroom-shaped dimer, composed of two transmembrane domain-swapped three-helix bundles and two intravirion domains. It regulates the localization of spike protein at cis-Golgi, the place of virus budding. It may function by binding the cytoplasmic C-terminus of the spike protein. The M protein can assemble into higher-order oligomers. It is the most abundant structural protein on the surface of the SARS-CoV-2 virus particle. This description may include information annotated by UniProt and/or Google AI.