Product Name:
SARS-CoV2_1b: NSP13 - 6713-6735
Product Number:
ab-nncov2-1b-3
Product Type Specific: SARS-CoV-2 virus NSP13 protein pan-specific antibody
Antibody Code: NNCOV2-1B-3
Antibody Target Type: Pan-specific
Protein UniProt: P0DTD1
Protein SigNET: SARS-CoV2_1b: NSP13 - 6713-6735
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human SARS-CoV2_1b
Antibody Immunogen Sequence: CSPFELEDFIPMDSTVKNYFITDAC
Antibody Immunogen Description: Corresponds to amino acid residues S6713 to A6735.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.65 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: SARS-CoV-2.
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Scientific Background: The SARS-CoV-2 virus Non-structural protein-1 (NSP13) gene encodes a helicase that functions in viral RNA synthesis. It is a multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. The activity of helicase is dependent on magnesium. It binds to host TBK1 and inhibits TBK1 phosphorylation; the interaction with TBK1 decreases IRF3 phosphorylation, which leads to reduced IFN-beta production. This description may include information annotated by UniProt and/or Google AI.