Product Name:
SARS-CoV2_1b: NSP15 -6053-6071
Product Number:
ab-nncov2-1b-2
Product Type Specific: SARS-CoV-2 virus NSP15 protein pan-specific antibody
Antibody Code: NNCOV2-1B-2
Antibody Target Type: Pan-specific
Protein UniProt: P0DTD1
Protein SigNET: SARS-CoV2_1b: NSP15 -6053-6071
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human SARS-CoV2_1b
Antibody Immunogen Sequence: CAPNNTDFSRVSAKPPPGDQF
Antibody Immunogen Description: Corresponds to amino acid residues A6053 to F6071.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.55 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: SARS-CoV-2.
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Scientific Background: The SARS-CoV-2 virus Non-structural protein-15 (NSP15) gene encodes a uridylate-specific endoribonuclease that plays a role in viral transcription/replication and prevention of the simultaneous activation of host cell dsRNA sensors, such as MDA5/IFIH1, OAS, and PKR. It likely acts by degrading the 5'-polyuridines generated during replication of the poly(A) region of viral genomic and subgenomic RNAs. It catalyzes a two-step reaction in which a 2'3'-cyclic phosphate (2'3'-cP) is first generated by 2'-O transesterification, which is then hydrolyzed to a 3'-phosphate (3'-P). If not degraded, poly(U) RNA would likely hybridize with poly(A) RNA tails and activate host dsRNA sensors. It may bind genomic dsRNA in association with the replication-transcription complex (RTC), and play a role in nsp12 discontinous transcription. This description may include information annotated by UniProt and/or Google AI.