Product Name:
SARS-CoV2_1a: NSP3 -876-890
Product Number:
ab-nncov2-1a-3
Product Type Specific: SARS-CoV-2 virus NSP3 protein pan-specific antibody
Antibody Code: NNCOV2-1A-3
Antibody Target Type: Pan-specific
Protein UniProt: P0DTC1
Protein SigNET: SARS-CoV2_1a: NSP3 -876-890
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human SARS-CoV2_1a
Antibody Immunogen Sequence: CADAVIKTLQPVSELL
Antibody Immunogen Description: Corresponds to amino acid residues A876 to L890.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: SARS-CoV-2.
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Scientific Background: The SARS-CoV-2 virus Non-structural protein-3 (NSP3) gene encodes a papain-like protease that is responsible for the cleavages located at the N-terminus of the replicase polyprotein. It participates together with NSP4 in the assembly of virally-induced cytoplasmic double-membrane vesicles that are necessary for viral replication. It antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. It also likely prevents host NF-kappa-B signaling. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both Lys-48- and Lys-63-linked polyubiquitin chains from cellular substrates. It preferentially cleaves ISG15 from antiviral protein IFIH1 (MDA5), but not RIGI. It can facilitate host ADP-ribosylation by ADP-ribose. It plays a role in the formation and maintenance of double membrane vesicles (DMVs) replication organelles. The DMVs are formed by NSP3 and NSP4, while NSP6 zippers ER membranes and connects to lipid droplets. This description may include information annotated by UniProt and/or Google AI.