Product Name:
SARS-CoV2_1a:NSP-2 -735-750
Product Number:
ab-nncov2-1a-2
Product Type Specific: SARS-CoV-2 virus NSP2 protein pan-specific antibody
Antibody Code: NNCOV2-1A-2
Antibody Target Type: Pan-specific
Protein UniProt: P0DTC1
Protein SigNET: SARS-CoV2_1a:NSP-2 -735-750
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human SARS-CoV2_1a
Antibody Immunogen Sequence: CAPKEIIFLEGETLPTE
Antibody Immunogen Description: Corresponds to amino acid residues A735 to E750.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.6 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: SARS-CoV-2.
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Scientific Background: The SARS-CoV-2 virus Non-structural protein-2 (NSP2) enhances mRNA repression of the 4EHP-GYF2 complex in the host, thereby inhibiting the antiviral response and facilitating SARS-CoV-2 replication. It possibly acts in cooperation with NSP1, which induces ribosome stalling on host mRNA, and triggers mRNA repression by the host 4EHP-GYF2 complex which is enhanced by NSP2. This description may include information annotated by UniProt and/or Google AI.