Product Name:
KDEL
Product Number:
ab-nn277-1
Target Full Name: Lys Asp Glu Leu peptide
Product Type Specific: KDEL pan-specific antibody
Antibody Code: NN277-1
Antibody Target Type: Pan-specific
Protein SigNET: KDEL
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: KDEL containing peptide immunogen
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.2, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence | Immunohistochemistry
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a KDEL proteins, GRP94, Grp78, PDI and calreticulin.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: A 1:1000 dilution of SPC-109 was sufficient for detection of KDEL-containing proteins in 20ug of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG as the secondary.
Antibody Cross Reactivity: May also immunoreact with ERp57 and ERp29.
Scientific Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This description may include information annotated by UniProt and/or Google AI.