Product Name:
KCNQ1
Product Number:
ab-nn274-1
Target Full Name: Potassium voltage-gated channel subfamily KQT member 1
Target Alias: ATFB1; ATFB3; FLJ26167; IKs producing slow voltage-gated potassium channel subunit alpha; IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1; Jervell and Lange-Nielsen syndrome 1; JLNS1; KCNA8; KCNA9; KCNQ1; KCNQ1_HUMAN; kidney and cardiac voltage dependend K+ channel; KQT-like 1; Kv1.9; Kv7.1; KVLQT1; long (electrocardiographic) QT syndrome; Ward-Romano syndrome 1; LQT; LQT1; Potassium voltage-gated channel KQT-like subfamily member 1; RWS; slow delayed rectifier channel subunit; SQT2; Voltage-gated potassium channel subunit Kv7.1; WRS
Product Type Specific: Potassium channel pan-specific antibody
Antibody Code: NN274-1
Antibody Target Type: Pan-specific
Protein UniProt: P51787
Protein SigNET: KCNQ1
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with human KCNQ1 N-terminus peptide
Antibody Immunogen Description: Corresponds to amino acid residues A2-L101.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium potency. Detects a ~75 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Chimpanzee | Rat | Mouse | Hamster
Antibody Positive Control: 1 µg/ml of SMC-307 was sufficient for detection of KCNQ1 in 10 µg of COS-1 cell lysate transiently expressing KCNQ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: High
Related Product 1: KCNQ2 pan-specific antibody (Cat. No.: AB-NN275-1)
Related Product 2: KCNQ4 pan-specific antibody (Cat. No.: AB-NN276-1)
Scientific Background: Kv7.1 (KvLQT1) is a potassium channel protein coded for by the gene KCNQ1. It is a voltage-gated potassium channel alpha-subunit crucial for regulating cardiomyocyte excitability and cardiac repolarization, and maintaining epithelial function. It forms the slow delayed rectifier current by assembling with KCNE1 beta subunits. It serves as a pore-forming subunit that creates an outward-directed potassium current, which triggers cardiac membrane repolarization to terminate the action potential. It is a tetramer with each subunit containing six transmembrane segments (S1-S6), including voltage-sensing domains (S1-S4) and a central pore domain (S5 & S6). It requires PIP2 (phosphatidylinositol 4,5-bisphosphate) to conduct current and is modulated by KCNE subunits (KCNE1–5), which modify the current kinetics. Protein kinase A (PKA) can stimulate Kv7.1 activity and affect its surface expression. Kv7.1 is present in the cell membranes of cardiac muscle tissue (primarily heart ventricles) and in inner ear neurons (1), stomach and colon among other tissues. In the cardiac cells, Kv7.1 mediates the IKs (or slow delayed rectifying K+) current that contributes to the repolarization of the cell, terminating the cardiac action potential and thereby the heart's contraction (2, 3). Loss-of-function mutations are linked to long QT syndrome (LQT1), causing arrhythmia. Gain-of-function mutations are linked to atrial fibrillation and short QT syndrome. This description may include information annotated by UniProt and/or Google AI.