Product Name:
KCNQ2
Product Number:
ab-nn275-1
Target Full Name: Potassium voltage-gated channel subfamily KQT member 2
Target Alias: BFNC; EBN1; ENB1; HNSPC; KCNA11; KQT like 2; KV7.2; KVEBN1. KvLQT2; voltage gated potassium channel subunit Kv7.2
Product Type Specific: Potassium channel pan-specific antibody
Antibody Code: NN275-1
Antibody Target Type: Pan-specific
Protein UniProt: O43526
Protein SigNET: KCNQ2
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with human KCNQ2 N-terminus peptide
Antibody Immunogen Description: Corresponds to amino acid residues M1-A59.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium-high potency. Detects a ~95 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Hamster.
Antibody Positive Control: 1 µg/ml of SMC-308 was sufficient for detection of KCNQ2 in 10 µg of COS-1 cell lysate transiently expressing KCNQ2 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Related Product 1: KCNQ1 pan-specific antibody (Cat. No.: AB-NN274-1)
Related Product 2: KCNQ4 pan-specific antibody (Cat. No.: AB-NN276-1)
Scientific Background: Kv7.2 (KvLQT2) (encoded by the KCNQ2 gene) is a voltage-gated potassium channel protein crucial for regulating neuronal excitability. It forms homo- or heterotetramers (mainly with Kv7.3) to produce the "M-current," a low-threshold, non-inactivating current that prevents neuronal hyperexcitability. Kv7.2 features six transmembrane segments (S1-S6), with a voltage-sensing domain (S1-S4) and a pore domain (S5 & S6). A large C-terminus is vital for assembly and trafficking. It becomes activated at subthreshold potentials (≥ -60 mV)) to stabilize the resting membrane potential. It requires phosphatidylinositol-4,5-bisphosphate (PIP2) to open the channel. Kv7.2 is highly expressed in the developing and adult brain, primarily found in neuronal cell bodies. KCNQ2 mutations are linked to Benign Familial Neonatal Convulsions (BFNC) (1) and early-onset epileptic encephalopathy. This description may include information annotated by UniProt and/or Google AI.