Product Name:
Aquaporin 3
Product Number:
ab-nn201-1
Target Full Name: Aquaporin 3
Target Alias: AQP3; AQP 3; AQP-3; Aqp3; AQP3_HUMAN; Aquaglyceroporin-3; Aquaporin 3 (GIL blood group); Aquaporin 3 (Gill blood group); GIL; Gill blood group
Product Type Specific: Aquaporin pan-specific antibody
Antibody Code: NN201-1
Antibody Target Type: Pan-specific
Protein UniProt: Q92482
Protein SigNET: Aquaporin 3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: C-terminal peptide based on rat aquaporin 3 (Uniprot ID P47862)
Antibody Immunogen Sequence: CHLEQPPPSTEAENVKLAHMKHKEQI
Antibody Immunogen Description: Corresponds to amino acid residues C267-I292.
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~31.5 kDa protein in cell and tissue lysates by Western blotting. May detect larger glycosylated ~35-50 kDa proteins.
Antibody Species Reactivity: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus
Antibody Positive Control: 0.5 µg/ml of SPC-504 was sufficient for detection of aquaporin 3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: Aquaporin 1 expression antibody (Cat. No.: AB-NN199-1)
Related Product 2: Aquaporin 2 expression antibody (Cat. No.: AB-NN200-1)
Related Product 3: Aquaporin 4 expression antibody (Cat. No.: AB-NN202-1)
Scientific Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin-3 (AQP3) functions as an aquaglyceroporin, transporting water, glycerol, and hydrogen peroxide (H2O2) across cell membranes, particularly in the skin, kidneys, and airways. It is crucial for skin hydration, wound healing, and cell proliferation, with high expression in basolateral cell membranes of kidney collecting duct cells of the kidney, regulating urine concentration by providing a pathway for water to exit these cells (3). AQP3 gene expression is not regulated by vasopressin (4). Its activity is regulated by pH, with lower pH (acidic) levels inhibiting its permeability. Deficiency of AQP3 leads to polyuria (excessive urine production). AQP3 is linked to cancer progression (such as in breast and gastric cancer) where it supports cell migration. It is also involved in skin inflammation, particularly in diseases like psoriasis and rosacea. This description may include information annotated by UniProt and/or Google AI.