Product Name:
Aquaporin 4
Product Number:
ab-nn202-1
Target Full Name: Aquaporin 4
Target Alias: AQP 4; AQP-4; AQP4; AQP4_HUMAN; Aquaporin type 4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; Mercurial-insensitive water channel; MGC22454; MIWC; WCH 4; WCH4
Product Type Specific: Aquaporin pan-specific antibody
Antibody Code: NN202-1
Antibody Target Type: Pan-specific
Protein UniProt: P55087
Protein SigNET: Aquaporin 4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: C-terminal peptide based on rat aquaporin 4 (Uniprot ID P47863)
Antibody Immunogen Sequence: CTKGSYMEVEDNRSQVETED
Antibody Immunogen Description: Corresponds to amino acid residues T273-D291.
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:400); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~35 kDa protein in cell and tissue lysates by Western blotting. May detect larger glycosylated ~50 kDa proteins.
Antibody Species Reactivity: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse
Antibody Positive Control: 0.5 µg/ml of SPC-505 was sufficient for detection of aquaporin 4 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: Aquaporin 1 expression antibody (Cat. No.: AB-NN199-1)
Related Product 2: Aquaporin 2 expression antibody (Cat. No.: AB-NN200-1)
Related Product 3: Aquaporin 3 expression antibody (Cat. No.: AB-NN201-1)
Scientific Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins (1, 2). Aquaporin-4 (AQP4) is the primary water-transporting protein in the mammalian central nervous system (CNS), forming highly selective bidirectional channels in the plasma membrane of astrocytes, particularly at the blood-brain barrier. AQP4 is up regulated by direct insult to the CNS (4). It facilitates rapid water movement, maintaining brain water homeostasis, AQP4 is constitutively expressed in the basolateral cell membrane of principal collecting duct cells in the kidney and provide a pathway for water to exit these cells (3). AQP4 is a homotetramer, consisting of four identical subunits, with a molecular mass of roughly 34-38 kDa per monomer. It exists as a transmembrane protein with six helical, membrane-spanning domains and two short helical segments creating a narrow, selective aqueous pore of ~2.8 Å in diameter. AQP4 is the target of autoantibodies in Neuromyelitis Optica (NMO), leading to inflammation in the brain and spinal cord ( 5, 6). It is critically involved in the development of brain edema (swelling) following injury, such as stroke or trauma. This description may include information annotated by UniProt and/or Google AI.