Product Name:
CaMKII pT286/pT287
Product Number:
ab-nk302-2
Target Full Name: Calcium/calmodulin-dependent protein kinase type II subunits
Target Alias: CaMK2; CaMK2A; CaMK2B; CaMK2D; CaMK2G; CAMKA
Product Type Specific: CaMK2a protein kinase pan-specific antibody
Antibody Code: NK302-2
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T286
Protein UniProt: Q9UQM7
Protein SigNET: CaMKII pT286/pT287
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Synthetic peptide
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation | ELISA
Antibody Dilution Recommended: WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a phosphorylated CaMKII from rat tissues. This antibody is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β).
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml was sufficient for detection of 0.2µg CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Antibody Specificity: High
Related Product 1: CaMK2a T286 phosphorylation antibody (Cat. No.: AB-PK555)
Related Product 2: CaMK2a Y230 phosphorylation antibody (Cat. No.: AB-PK875)
Related Product 3: CaMKII (Alpha expression antibody (Cat. No.: AB-NK302-1)
Related Product 4: CaMK2b expression antibody (Cat. No.: AB-NK018-3)
Related Product 5: CaMK2g expression antibody (Cat. No.: AB-NK020-1)
Related Product 6: CaMK2d expression antibody (Cat. No.: AB-NK019-3)
Related Product 7: CAMK2Subtide - CaMK2a protein kinase substrate peptide
Related Product 8: Syntide-2 KinSub - Syntide-2 protein kinase substrate peptide
Scientific Background: CaMK2a (CaMKII) is a protein-serine/threonine kinase of the CaMK group and CaMK2 family. It is a calcium/calmodulin-activated protein kinase involved in neurotransmitter release and long term potentiation in neurons. It is highly abundant in the brain, particularly at excitatory synapses in the postsynaptic density. It acts as a key mediator of synaptic plasticity, learning, and memory by regulating long-term potentiation (LTP) and activating upon increased calcium levels. Autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state. It is also involved in regulation of cell cycle progression, T-cell activation, and cardiac function. CaMK2a resides in large multi-oligomeric protein complexes (12 subunits) with other CaMK2 subunits (beta, gamma and delta). Mutations in the CAMK2A gene are linked to neurodevelopmental disorders, specifically autosomal dominant and recessive intellectual disabilities. This description may include information annotated by UniProt and/or Google AI.