Product Name:
Phosphotyrosine
Product Number:
ab-cn005-2
Target Full Name: Phosphotyrosine sites in proteins
Target Alias: PhosphoTyrosine (pY); Phospho-Tyrosine
Product Type Specific: Generic phosphotyrosine-specific pan-specific antibody
Antibody Code: CN005-2
Antibody Target Type: Pan-specific
Protein SigNET: Phosphotyrosine
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Phosphotyrosine, alanine and glycine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin with 1-ethyl-3-(3'-dimentrylaminopropyl) carbodiimide
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium potency. Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in both unstimulated and stimulated cell lysates.
Antibody Positive Control: 1 µg/ml of SMC-174 was sufficient for detection of phosphorylated tyrosine residues in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Cross Reactivity: Does not cross-react with phosphoserine or phosphothreonine.
Related Product 1: PYK generic phosphotyrosine-specific antibody (Cat. No.: AB-PG001)
Related Product 2: PYK-Ultra generic phosphotyrosine-specific antibody (Cat. No.: AB-PG001U)
Scientific Background: Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (3). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (4-6). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (7). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (8). This description may include information annotated by UniProt and/or Google AI.