Product Name:
Raf1 (256-262) pS259
Product Number:
pe-04ayc00
Peptide Name: Raf1 (256-262) pS259
Product Use: This phosphopeptide may be useful as a substrate for screening the phosphatase activity of protein phosphatases and for epitope mapping of phosphosite-specific antibodies. The peptide sequence is located in the region between the C1_1 and kinase catalytic domains. This is the major in vivo phosphorylation site in Raf1. Phosphorylation inhibits phosphotransferase activity, reduces H-Ras-1 and PPP2CB binding, and induces binding of 14-3-3-beta and zeta. It appears to necessary for PKC-alpha activation of Raf1.
Peptide Production Method: Solid-phase peptide synthesis
Peptide Origin: Homo sapiens
Peptide Sequence: RST-pS-TPN
Peptide Modifications N Terminus: Free amino
Peptide Modifications C Terminus: Amide
Peptide Modifications Other: Phosphorylated
Peptide Molecular Mass Calculated: 840.77 Da
Peptide Purity Percent after Synthesis and Purification: >70
Peptide Appearance: White powder
Peptide Form: Solid
Storage Conditions: -20°C