Product Name:
HeLa-EGF Lysate
Product Number:
lw0303s
Target Full Name: Human epidermoid carcinoma HeLa cells - EGF-treated - Whole lysate
Product Type Specific: Whole lysate in SDS-PAGE sample buffer
Production Method: HeLa cell lysates were cultivated to 70-90% confluency and deprived of serum for 18-20 hours and then treated for 100 ng/ml epidermal growth factor (EGF) for 10 minutes prior to harvesting. Lysates were prepared from scrapped cells that were homogenized by sonication in buffer formulated with 1% Triton X-100, 60 mM ß-glycerophosphate, pH 7.2, 20 mM MOPS, 20 mM sodium pyrophosphate, 30 mM sodium fluoride, 5 mM EDTA, 3 mM benzamidine, 2 mM EGTA, 1 mM sodium orthovanadate, 1 mM phenylmethylsulfonylfluoride, 1 mM dithiothreitol, 10 µM leupeptin, and 5 µM pepstatin. Lysates were prepared following sonication and 30 min ultracentrifugation at 100,000 rpm. Lysates were further diluted in SDS-PAGE sample buffer at a final concentration of 2 mg/ml.
Storage Conditions: -70°C
Storage Stability: 1 year at -70°C
Product Use: For testing antibodies by immunoblotting
Related Product 1: Cat. No.: LW0303F - HeLa whole lysate from EGF-treated cells (3 mg/ml)
Related Product 2: Cat. No.: LW0301F - HeLa whole lysate from untreated cells (3 mg/ml)
Related Product 3: Cat. No.: LW0301S - HeLa whole lysate from untreated cells (2 mg/ml) in SDS-PAGE sample buffer
Related Product 4: Cat. No.: LW0302S - HeLa whole lysate from anisomycin-treated cells (2 mg/ml) in SDS-PAGE sample buffer
Related Product 5: Cat. No.: LW0302F - HeLa whole lysate from anisomycin-treated cells (3 mg/ml)
Related Product 6: Cat. No.: LW0311S - HeLa whole lysate from PMA-treated cells (2 mg/ml) in SDS-PAGE sample buffer
Related Product 7: Cat. No.: LW0311F - HeLa whole lysate from PMA-treated cells (3 mg/ml)