Product Name:
HEK293+Forskolin Cytosol
Product Number:
lc0205f
Target Full Name: Human Embryonic Kidney-293 cells - Forskolin-treated - Cytosolic lysate
Product Type Specific: Cytosolic lysate
Production Method: HEK293 cells were cultivated to 80% confluency and deprived of serum for 18 hours and then treated with 150 nM forskolin for 15 minutes prior to harvesting. Lysates were prepared from scrapped cells that were homogenized by sonication in buffer formulated with 60 mM ß-glycerophosphate, pH 7.2, 20 mM MOPS, 20 mM sodium pyrophosphate, 30 mM sodium fluoride, 5 mM EDTA, 3 mM benzamidine, 2 mM EGTA, 1 mM sodium orthovanadate, 1 mM phenylmethylsulfonylfluoride, 1 mM dithiothreitol, 10 µM leupeptin, and 5 µM pepstatin A. Cytosolic lysates were prepared following sonication and 30 min ultracentrifugation at 100,000 rpm. Lysates were further diluted in homogenizing buffer at a final concentration of 3 mg/ml.
Storage Conditions: -70°C
Storage Stability: 1 year at -70°C
Product Use: For testing antibodies by immunoprecipitation or immunoblotting, and for assays of enzymes
Related Product 1: Cat. No.: LC0205S - HEK293 cytosolic lysate from forskolin-treated cells (2 mg/ml) in SDS-PAGE sample buffer
Related Product 2: Cat. No.: LC201S - HEK293 cytosolic lysate from untreated cells (2 mg/ml) in SDS-PAGE sample buffer
Related Product 3: Cat. No.: LC201F - HEK293 cytosolic lysate from untreated cells (3 mg/ml)