Product Name:
KAM-880
Product Number:
ar_kam900p
Target Full Name: Kinex™ KAM-880 Antibody Microarray
Product Type Specific: Antibody microarray
Antibody Modification: Antibody microarray
Storage Conditions: Upon receipt, the KAM-880 chip and dye-mix should be kept frozen at -20°C. Other components can be stored at 4°C for 1 month. For longer storage, the G25 spin-column and incubation chamber can be left at 4°C, but the other components should be frozen at -20°C.
Storage Stability: The KAM-880 Kit can be stored for at least 1 year if frozen at -20°C (G25 spin column and incubation chamber should not be frozen).
Scientific Background: High content antibody microarrays are powerful tools that permit the mass profiling of hundreds of diverse proteins for alterations in protein levels, post-translational modifications and protein-protein interactions. Kinexus offers a chemical cleavage step that reduces the occurence of protein-protein interactions. With their high sensitivities, only 25-50 µg amounts of cell or tissue lysate proteins are required. With the KAM-880 chip, highly validated antibodies are printed on to the surface of a glass slide. Lysates proteins are pre-labeled with a proprietary mixture of fluorescent dyes, and subsequently captured on the microarray. The intensity of the fluorescent signal at each antibody spot is a measure of how much protein was bound. Due to the possibilities of antibody cross-reactivities and protein-protein interactions in diverse cell/tissue types, the leads should be followed by Western blotting. Kinexus offers a parallel multi-immunoblotting service to confirm the results of our KAM antibody microarrays. Over 4500 antibody microarray analyses performed to date. Open access in KiNET-AM (www.kinet-am.ca) to full experimental data from over 2000 analyses available online for comparisons. Over 150 scientific peer-reviewed papers published using our antibody microarrays.