Product Name:
PTEN-pY336
Product Number:
ab-pp556
Target Full Name: Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and protein phosphatase and tensin homologue deleted on chromosome 10
Target Alias: BZS; MHAM; MMAC1; Mutated in multiple advanced cancers 1; Phosphatase and tensin; Phosphatase and tensin homolog gene; Protein-tyrosine phosphatase PTEN; PTEN1; TEP1
Product Type Specific: PTEN protein phosphatase phosphosite-specific antibody
Antibody Code: PP556
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y336
Protein UniProt: P60484
Protein SigNET: PTEN
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PTEN
Antibody Immunogen Sequence: KANR(pY)FSP(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues K332 to P339. Y336 phosphorylation stimulates PTEN phosphatase activity and protein stability, which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. Frk binds (via its SH3-domain) with PTEN. This site was not identified as a site of in vivo phosphorylation of PTEN based mass spectrometry reports recorded in PhosphoSitePlus. PTEN is known to be phosphorylated at this site in vitro by Frk.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.35 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Rat | Mouse | Platypus.
Related Product 1: PTEN Y315 phosphorylation antibody (Cat. No.: AB-PP531)
Related Product 2: PTEN-1 pan-specific antibody (Cat. No.: AB-NP023-3)
Related Product 3: PTEN-2 pan-specific antibody (Cat. No.: AB-NP023-4)
Related Product 4: PTEN-3 pan-specific antibody (Cat. No.: AB-NP023-5)
Scientific Background: PTEN is a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins, and also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for the tumour suppressor function of PTEN. It antagonizes the PI3K-AKT/PKB signalling pathway by dephosphorylating phosphoinositides, and inhibits cell cycle progression and cell survival. The unphosphorylated form of PTEN cooperates with AIP1 to suppress Akt1 activation. It dephosphorylates tyrosine-phosphorylated focal adhesion kinase (FAK) and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. It plays a role as a key modulator of the AKT-mTOR signalling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis. It acts as negative regulator of insulin signalling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumour suppressive ability. In motile cells, PTEN suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement. This description may include information annotated by UniProt and/or Google AI.