Product Name:
PTPRE-pY696
Product Number:
ab-pp554
Target Full Name: Receptor-type tyrosine-protein phosphatase epsilon
Target Alias: EC 3.1.3.48; PTPE; PTP-epsilon; R-PTP-epsilon
Product Type Specific: PTPRE protein phosphatase phosphosite-specific antibody
Antibody Code: PP554
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y696
Protein UniProt: P23469
Protein SigNET: PTPRE
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PTPRE
Antibody Immunogen Sequence: FSD(pY)ANF(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues F693 to F699. The effect of Y696 phosphorylation is unclear, but it is the major site of phosphorylation by mass spectrometry (≥605 reports recorded in PhosphoSitePlus). PTPRE is known to be phosphorylated at this site in vitro by ErbB2 (HER2).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.6 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish.
Related Product 1: PTPRA Y798 phosphorylation antibody (Cat. No.: AB-PP521)
Related Product 2: PTPRB Y1981 phosphorylation antibody (Cat. No.: AB-PP522)
Related Product 3: PTPRF Y1621 phosphorylation antibody (Cat. No.: AB-PP523)
Related Product 4: PTPRK Y858 phosphorylation antibody (Cat. No.: AB-PP539)
Related Product 5: PTPRK Y916 phosphorylation antibody (Cat. No.: AB-PP524)
Related Product 6: PTPRM Y929 phosphorylation antibody (Cat. No.: AB-PP526)
Related Product 7: PTRF Y308 phosphorylation antibody (Cat. No.: AB-PN533)
Related Product 8: PTRF Y308 phosphorylation antibody (Cat. No.: AB-PN646)
Scientific Background: PTPRE (Protein tyrosine phosphatase receptor type E) is an enzyme that belongs to the protein-tyrosine phosphatase family, Receptor class 4 subfamily. It functions as a key protein phosphatase that regulates cell growth, differentiation, and oncogenic transformation. It exists in transmembrane (Isoform 1) and cytoplasmic forms (Isoform 2). Isoform 1 plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. It also plays a role in osteoclast formation and function. The transmembrane Receptor-type (RPTPe) features a short extracellular domain, a single transmembrane region, and two tandem intracytoplasmic catalytic domains. Isoform 2 acts as a negative regulator of insulin receptor (IR) signaling in skeletal muscle. It regulates insulin-induced tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate 1 (IRS-1), phosphorylation of protein kinase B and glycogen synthase kinase-3 and insulin induced stimulation of glucose uptake. Isoform 2 (Non-receptor type) possesses a distinct hydrophilic N-terminus. Both isoforms act as negative regulators of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca(2+). Its activity is regulated by dimerization, often induced by oxidative stress, and by phosphorylation on specific residues. It is ubiquitously expressed, with high expression in immune cells and additional presence in endothelial cells. PTPRE acts as an oncogene in specific cancers, including retinoblastoma and thyroid carcinoma, by enhancing proliferation, promoting tumour growth, and inhibiting apoptosis. It is also crucial for osteoclast function. It is highly xpressed in giant cell tumours (osteoclastoma rich in multinucleated osteoclastic cells). This description may include information annotated by UniProt and/or Google AI.