Product Name:
PPP6C-pY261
Product Number:
ab-pp553
Target Full Name: Serine/threonine-protein phosphatase 6 catalytic subunit
Target Alias: EC 3.1.3.16; PP6; PPP6
Product Type Specific: PPP6C protein phosphatase phosphosite-specific antibody
Antibody Code: PP553
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y261
Protein UniProt: O00743
Protein SigNET: PPP6C
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PPP6C
Antibody Immunogen Sequence: CSAPN(pY)SYR
Antibody Immunogen Description: Corresponds to amino acid residues S257 to R264. The effect of Y261 phosphorylation is unclear. This is the major site of in vivo phosphorylation of PPP6C (≥11 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.6 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Chicken | Frog | Zebra fish | Fruit fly | Honey bee | Nematode worm | Sea urchin | Poplar tree | Thale cress | Budding yeast | Red bread mold.
Related Product 1: PPP3CC T304 phosphorylation antibody (Cat. No.: AB-PP506)
Related Product 2: PP5 (PPT) expression antibody (Cat. No.: AB-NP021-3)
Related Product 3: PP5 (PPT) expression antibody (Cat. No.: AB-NP021-4)
Related Product 4: PPP5C S463 phosphorylation antibody (Cat. No.: AB-PP507)
Scientific Background: PPP6C (Catalytic subunit of protein phosphatase 6) is a component of a signalling pathway that mediates control of cell cycle progression at the G1 to S phase (via regulation of cyclin D1 production) in response to IL2. It also targets MAP3K7 for dephosphorylation in response to IL1. It also dephosphorylates S435 of CGAS, which inactivates the CGAS-STING pathway that regulates innate immunity. This description may include information annotated by UniProt and/or Google AI.