Product Name:
PPP1R1A-pT35
Product Number:
ab-pp548
Target Full Name: Protein phosphatase 1 regulatory subunit 1A
Target Alias: I-1; Inhibitor 1; IPP1; PPR1A
Product Type Specific: PPP1R1A protein phosphatase phosphosite-specific antibody
Antibody Code: PP548
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T35
Protein UniProt: Q13522
Protein SigNET: PPP1R1A
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PPP1R1A
Antibody Immunogen Sequence: CRRRP(pT)PAT
Antibody Immunogen Description: Corresponds to amino acid residues R31 to T38. T35 phosphorylation stimulates phosphatase activity through inhibition of this inhibitor. This is a minor in vivo phosphorylation site in PPP1R1A based on ≥4 mass spectrometry reports recorded in PhosphoSitePlus. PPP1R1A is known to be phosphorylated at this site in vitro by PKACa (PRKACA).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Frog | Zebra fish.
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Scientific Background: PPP1R1A (Protein Phosphatase 1 Regulatory Subunit 1A; Inhibitor-1) is a 20-28 kDa cytoplasmic protein that inhibits Protein Phosphatase 1 (PP1), and regulates cell signaling, glycogen metabolism, and cardiac contractility. Its inhibitory activity towards PP1 catalytic activity is increased by cAMP-mediated phosphorylation at Thr-35 and decreased by the protein phosphatase calcineurin (PP2B). It does not inhibit type-2 phosphatases. It is highly expressed in tissues like the heart and pancreas. It is implicated in Ewing sarcoma, where it is upregulated to promote tumour growth. It is also identified as a potential biomarker in diabetes (pancreatic damage). This description may include information annotated by UniProt and/or Google AI.