Product Name:
PPP1R14B-pT57
Product Number:
ab-pp545
Target Full Name: Protein phosphatase 1 regulatory subunit 14B
Target Alias: PHI-1; Phospholipase C beta 3 neighbouring gene protein; Phospholipase C beta-3 neighboring gene protein; PLCB3N; PNG; PP14B; Protein phosphatase 1, regulatory (inhibitor) subunit 14B; SOM172
Product Type Specific: PPP1R14B protein phosphatase phosphosite-specific antibody
Antibody Code: PP545
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: T57
Protein UniProt: Q96C90
Protein SigNET: PPP1R14B
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PPP1R14B
Antibody Immunogen Sequence: GKV(pT)VKY(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues G54 to Y60. T57 phosphorylation stimulates phosphatase inhibitory activity. This is a minor in vivo phosphorylation site in PPP1R14B based on ≥5 mass spectrometry reports recorded in PhosphoSitePlus. PPP1R14B is known to be phosphorylated at this site in vitro by ILK, PKCa (PRKCA), PKCd (PRKCD), and ROCK2 (ROK-alpha).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.6 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Chicken.
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Scientific Background: PPP1R14B (Protein Phosphatase 1 regulatory inhibitor subunit 14B; PHI-1) is a cytoplasmic protein that acts as a phosphorylation-dependent inhibitor of Protein Phosphatase-1 (PP1). It regulates cellular signaling, smooth muscle contraction, and cytoskeletal dynamics. It functions as a inhibitor of PP1 holoenzymes. Its inhibitory activity increases over 50-fold when phosphorylated. It plays a significant role in regulating cellular homeostasis. It has also been shown to activate the AKT/NF-κB signaling pathway. PPP1R14B is frequently upregulated in various cancers (e.g., hepatocellular carcinoma, triple-negative breast cancer, lung adenocarcinoma). High expression levels are associated with poor prognosis and survival in many cancer types. This description may include information annotated by UniProt and/or Google AI.