Product Name:
PPP1R12A-pY496
Product Number:
ab-pp543
Target Full Name: Protein phosphatase 1 regulatory subunit 12A
Target Alias: MBS; Myosin phosphatase-targeting subunit 1; MYPT1
Product Type Specific: PPP1R12A protein phosphatase phosphosite-specific antibody
Antibody Code: PP543
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y496
Protein UniProt: O14974
Protein SigNET: PPP1R12A
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PPP1R12A
Antibody Immunogen Sequence: CGRLA(pY)VAP
Antibody Immunogen Description: Corresponds to amino acid residues R493 to P499. The effect of MYPT1 Y496 phosphorylation is unclear. This is a major in vivo phosphorylation site in MYPT1 based on ≥439 mass spectrometry reports recorded in PhosphoSitePlus. PPP1R12A is known to be phosphorylated at this site in vitro by ILK.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken | Frog.
Related Product 1: PPP1R11 Y64 phosphorylation antibody (Cat. No.: AB-PN532)
Related Product 2: PPP1R12A Y766 phosphorylation antibody (Cat. No.: AB-PP544)
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Related Product 4: PPP1R14B T57 phosphorylation antibody (Cat. No.: AB-PP545)
Related Product 5: PPP1R14B Y29 phosphorylation antibody (Cat. No.: AB-PP546)
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Related Product 7: PPP1R1A T35 phosphorylation antibody (Cat. No.: AB-PP548)
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Scientific Background: PPP1R12A (Protein phosphatase 1 regulatory subunit 12A; MYPT1) is a 115 kDa protein that acts as the regulatory targeting subunit of myosin light chain phosphatase (MLCP) with the catalytic subunit (PP1cδ) to modulate phosphatase activity. It crucially regulates vascular tone, smooth muscle contraction, cell migration, and cytoskeletal dynamics by controlling myosin light chain phosphorylation. It mediates binding to myosin. As part of a PPP1C complex, it involved in dephosphorylation of PLK1. It is capable of inhibiting HIF1AN-dependent suppression of HIF1A activity. Rho-associated kinase (ROCK) inactivates the myosin phosphatase complex by phosphorylating PPP1R12A, leading to increased myosin light chain (MLC) phosphorylation and contraction. It plays a significant role in various cell functions beyond muscle contraction, including the potential to influence the development of insulin resistance. It is primarily located in the cytoplasm, often associated with actomyosin filaments and stress fibers. It is expressed in smooth muscle cells and striated muscles (specifically in type 2a fibers). This description may include information annotated by UniProt and/or Google AI.