Product Name:
PPP1CA-pY134+pY137
Product Number:
ab-pp541
Target Full Name: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
Target Alias: EC 3.1.3.16; PP1A; PP-1A; PPP1A; Protein phosphatase 1, catalytic subunit, alpha isoform; Serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit; Serine/threonine protein phosphatase PP1-alpha 1, catalytic subunit
Product Type Specific: PPP1CA protein phosphatase phosphosite-specific antibody
Antibody Code: PP541
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y134+Y137
Protein UniProt: P62136
Protein SigNET: PPP1CA
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PPP1CA
Antibody Immunogen Sequence: NRI(pY)GF(pY)DE(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues N131 to E139. The effects of Y134 and Y137 phosphorylation are unclear. These are minor sites of in vivo phosphorylation based on ≥3 and ≥23 mass spectrometry reports, respectively, recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.45 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Platypus | Chicken | Frog | Zebra fish | Fruit fly | Honey bee | Neamtode worm | Sea urchin | Maize | Thale cress | Budding yeast | Red bread mold.
Related Product 1: PPM1B Y367 phosphorylation antibody (Cat. No.: AB-PP540)
Related Product 2: PPP1CA Y306 phosphorylation antibody (Cat. No.: AB-PP542)
Related Product 3: PPP1CB T66 phosphorylation antibody (Cat. No.: AB-PP502)
Related Product 4: PPP1CB Y306 phosphorylation antibody (Cat. No.: AB-PP530)
Related Product 5: PPP2CA Y284 phosphorylation antibody (Cat. No.: AB-PP549)
Related Product 6: PPP2CA T646 phosphorylation antibody (Cat. No.: AB-PP504)
Related Product 7: PPP2CB T304 phosphorylation antibody (Cat. No.: AB-PP505)
Related Product 8: PPPM1A Y119 phosphorylation antibody (Cat. No.: AB-PP508)
Scientific Background: PPP1CA (Protein Phosphatase 1 Catalytic Subunit Alpha) is a protein-serine/threonine phosphatase that belongs to the PPP phosphatase family, PP-1 subfamily. As a broad specificity phosphatase, it regulates diverse processes like cell division, metabolism including glycogen synthesis and breakdown, muscle contraction, transcription and synaptic plasticity. It exists as a ~37 kDa catalytic subunit, widely expressed in tissues, and forms specific complexes with >200 regulatory proteins to control hundreds of targets. These include substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase 2 and CENPA. It is a component of the PTW/PP1 phosphatase complex, which functions in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. It regulates NEK2 function in terms of its kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. It is a regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In concert with CSNK1D and CSNK1E, it determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. It can directly dephosphorylate CSNK1D and CSNK1E. It dephosphorylates the 'Ser-418' residue of FOXP3 and inactivates it in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby rendering Treg cells functionally defective. It dephosphorylates the 'Ser-139' residue of ATG16L1 causing dissociation of ATG12-ATG5-ATG16L1 complex, thereby inhibiting autophagy. PPP1CA is necessary for alphaviruses replication. Dysregulation is linked to cardiac hypertrophy, heart failure, and cancer, where abnormal activity affects tumour-related proteins. This description may include information annotated by UniProt and/or Google AI.