Product Name:
PPM1B-pY367
Product Number:
ab-pp540
Target Full Name: Protein phosphatase 1B; Protein phosphatase 2C isoform beta
Target Alias: PP2C-beta, PP2CB, Protein phosphatase 2C isoform beta
Product Type Specific: PPPM1B protein phosphatase phosphosite-specific antibody
Antibody Code: PP540
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y367
Protein UniProt: O75688
Protein SigNET: PPM1B
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PPM1B
Antibody Immunogen Sequence: CIEAV(pY)SRL
Antibody Immunogen Description: Corresponds to amino acid residues I363 to L370. The effect of PPM1B Y367 phosphorylation is unclear. This is one of the major sites of in vivo phosphorylation of PPPM1B based on ≥15 mass spectrometry reports recorded in PhosphoSitePlus. PPPM1B is known to be phosphorylated at this site in vitro by Abl1.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.7 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Rat | Mouse.
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Scientific Background: PPM1B (Protein Phosphatase, Mg2+/Mn2+ Dependent 1B; PP2C-beta) is a 36.3 kDa, cytoplasmic member of the PP2C family of serine/threonine protein phosphatases. PPM1B requires metal ions (Mg2+ or Mn2+) for its enzymatic activity. It has broad substrate specificity, targeting proteins involved in cell cycle control (such as CDK2 and CDK6) and energy metabolism. It functions as a negative regulator of cell stress response pathways, cell cycle progression, and inflammatory responses. PPM1B is heavily involved in mediating dephosphorylation and inactivation of key signaling molecules, including I-kappa-B kinase (IKK) and TBK1. It plays a crucial role in terminating Tumour Necrosis Factor-alpha (TNF-alpha)-induced NF-kappaB activation by inactivating IKBKB/IKKB. It also acts as a TBK1 phosphatase, regulating IRF3 activation and IFN-beta gene expression. PPM1B targets substrate protein dephosphorylation (e.g., PRKAA1/PRKAA2, components of AMPK signaling) to regulate energy metabolism. It interacts with Groucho 4 and is localized to DNA in a Groucho-dependent manner, requiring phosphatase activity for transcriptional silencing. It is particularly abundant in the heart and skeletal muscle. Overexpression of PPM1B is reported to cause cell-growth arrest or cell death. Dysfunction of PPM1B contributes to various diseases, including 2P21 Microdeletion Syndrome and Hypotonia-Cystinuria Syndrome. It is considered a potential drug target for treating cancers and inflammatory diseases. This description may include information annotated by UniProt and/or Google AI.