Product Name:
PTPN6-pY564
Product Number:
ab-pp538
Target Full Name: Tyrosine-protein phosphatase non-receptor type 6
Target Alias: 70Z-SHP; EC 3.1.3.48; HCP; HCPH; PTN6; PTP1C; SH-PTP1
Product Type Specific: PTPN6 protein phosphatase phosphosite-specific antibody
Antibody Code: PP538
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y564
Protein UniProt: P29350
Protein SigNET: PTPN6
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PTPN6
Antibody Immunogen Sequence: EDV(pY)ENL(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues E561 to L567. Y564 phosphorylation stimulates phosphatase activity, and induces interaction with 3BP2, Grb2, and PIK3R1. This is the major site of in vivo phosphorylation of PTPN6 (≥783 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). PTPN6 is known to be phosphorylated at this site in vitro by Lck, Lyn, and Src.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.35 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Zebra fish.
Scientific Background: PTPN6 (Protein Tyrosine Phosphatase Non-Receptor Type 6; SHP-1) belongs to the protein-tyrosine phosphatase family, Non-receptor class 2 subfamily. It is a critical phosphatase primarily expressed in hematopoietic cells that acts as a key regulator of signaling pathways. It interacts with substrates to regulate cell growth, differentiation, and inflammatory responses. PTPN6 possesses two tandem N-terminal Src homolog (SH2) domains, a catalytic domain, and a C-terminal tail. These SH2 domains allow it to bind to phosphotyrosine residues on target proteins. It plays a pivotal role in regulating the inflammatory process, with PTPN6 deficiency leading to increased inflammation. It functions as a negative regulator of JAK/STAT signaling, particularly in immune cells. It inhibits various pathways, including caspase-8-dependent apoptosis, p38 MAPK signaling, and necroptosis. Together with MTUS1, it induces UBE2V2 expression upon angiotensin II stimulation. It is predominantly found in hematopoietic cells, but also present in some epithelial cells. Isoform 1 is expressed in hematopoietic cells, whereas Isoform 2 is expressed in non-hematopoietic cells. It act as a tumour suppressor protein, and PTPN6 is frequently silenced in hematological cancers such as Acute Myeloid Leukemia (AML), and its loss is associated with increased malignancy. PTPN6 acts as a dendritic cell intrinsic checkpoint, influencing tumour immunity and NK cell function. Impairment of PTPN6 is linked to neutrophilic dermatosis and systemic inflammation. This description may include information annotated by UniProt and/or Google AI.