Product Name:
PTPN12-pS435
Product Number:
ab-pp536
Target Full Name: Protein-tyrosine phosphatase, non-receptor type 12
Target Alias: EC 3.1.3.48; Protein-tyrosine phosphatase G1; PTN12; PTNC; PTPG1; PTP-PEST
Product Type Specific: PTPN12 protein phosphatase phosphosite-specific antibody
Antibody Code: PP536
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S435
Protein UniProt: Q05209
Protein SigNET: PTPN12
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PTPN12
Antibody Immunogen Sequence: RNL(pS)FEI(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues R432 to I438. The effect of PTPN12 S435 phosphorylation is unclear. This is the major site of in vivo phosphorylation of PTPN12 (≥437 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). PTPN12 is known to be phosphorylated at this site in vitro by PKACa (PRKACA), and PKCa (PRKCA).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platypus | Chicken.
Related Product 1: PTPN12 Y62 phosphorylation antibody (Cat. No.: AB-PP513)
Related Product 2: PTPN11 Y62 phosphorylation antibody (Cat. No.: AB-PP512)
Related Product 3: PTPN11 Y546 phosphorylation antibody (Cat. No.: AB-PP534)
Related Product 4: PTPN11 Y584 phosphorylation antibody (Cat. No.: AB-PP535)
Related Product 5: https://kinexus-ca.myshopify.com/products/AB-PP514
Scientific Background: PTPN12 (Protein tyrosine phosphatase non-receptor type 12; PTP-PEST) is a ubiquitously expressed cytosolic phosphatase that belongs to the protein-tyrosine phosphatase family, Non-receptor class 4 subfamily. It regulates cell growth, cytoskeletal structure and adhesion, differentiation, and motility by dephosphorylating targets such as ErbB2 (at Tyr-1112, Tyr-1196 and Tyr-1248), c-ABL, FAK, and p130Cas. It is involved in T-cell regulation, specifically in secondary responses and promoting the formation of cell homoaggregates. It possesses an N-terminal PTP catalytic domain and a C-terminal PEST motif that mediates protein-protein interactions. As a tumour suppressor, it inhibits oncogenic transformation. It acts as a negative regulator of tyrosine kinases by removing phosphate groups, influencing cell migration, adhesion, and proliferation. PTPN12 has been found to suppress the progression of cancers such as hepatocellular carcinoma and osteosarcoma by targeting receptor tyrosine kinases. Reduced PTPN12 expression is associated with cancer metastasis and poor prognosis in various cancers, including non-small cell lung cancer and nasopharyngeal carcinoma. This description may include information annotated by UniProt and/or Google AI.