Product Name:
PTPN11-pY546
Product Number:
ab-pp534
Target Full Name: Tyrosine-protein phosphatase non-receptor type 11
Target Alias: BPTP3; EC 3.1.3.48; NS1; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; Protein-tyrosine phosphatase, non-receptor 11; PTN11; PTP1D; PTP-1D; PTP2C; PTP-2C; SHP2; SHPTP2; SH-PTP2; SH-PTP3; Syp
Product Type Specific: PTPN11 protein phosphatase phosphosite-specific antibody
Antibody Code: PP534
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y546
Protein UniProt: Q06124
Protein SigNET: PTPN11
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PTPN11
Antibody Immunogen Sequence: KGHE(pY)TNI(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues K542 to I549. Y542 phosphorylation stimulates phosphatase activity. This is a major site of in vivo phosphorylation of PTPN11 (≥453 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). PTPN11 is known to be phosphorylated at this site in vitro by Abl1, ALK, Fyn, and PDGFRB.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Mouse | Chicken | Frog | Fruit fly.
Related Product 1: PTPN11 Y62phosphorylation antibody (Cat. No.: AB-PP512)
Related Product 2: PTPN11 Y584 phosphorylation antibody (Cat. No.: AB-PP535)
Related Product 3: PTPN12 Y62 phosphorylation antibody (Cat. No.: AB-PP513)
Related Product 4: PTPN12 S435 phosphorylation antibody (Cat. No.: AB-PP536)
Related Product 5: PTPN14 S39 phosphorylation antibody (Cat. No.: AB-PP514)
Scientific Background: PTPN11 (SHP-2) is ubiquitously expressed non-receptor protein tyrosine phosphatase that belongs to the protein-tyrosine phosphatase family, Non-receptor class 2 subfamily. It is regulating cellular growth, differentiation, and migration through the RAS/MAPK signaling pathway. As a protein tyrosine phosphatase (PTP), SHP-2 acts to remove phosphate groups from tyrosine residues, modulating signals from growth factor and cytokine receptors. It is critical for embryonic development and hematopoietic cell function. This protein contains two tandem Src homology-2 (SH2) domains that regulate its activity by enabling interaction with substrates and switching between auto-inhibited and active conformations. SHP-2 is essential for activating the RAS-MAPK-ERK pathway, which drives cell proliferation and differentiation. It dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity. PTPN11 also dephosphorylates GAB1, ARHGAP35, EGFR and CDC73. Germline mutations, often leading to increased phosphatase activity, causing the developmental disorder Noonan syndrome. PTPN11 often acting as a proto-oncogene where gain-of-function mutations cause Noonan syndrome, LEOPARD syndrome, and various leukemias. Somatic mutations are linked to juvenile myelomonocytic leukemia (JMML), acute myeloid leukemia (AML), and other solid tumours. This description may include information annotated by UniProt and/or Google AI.