Product Name:
PPP1CB-pY306
Product Number:
ab-pp530
Target Full Name: Serine/threonine-protein phosphatase PP1-beta catalytic subunit
Target Alias: EC 3.1.3.16; PP1B; PP-1B
Product Type Specific: PPP1CB protein phosphatase phosphosite-specific antibody
Antibody Code: PP530
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y306
Protein UniProt: P62140
Protein SigNET: PPP1CB
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PPP1CB
Antibody Immunogen Sequence: CAKYQ(pY)GGL
Antibody Immunogen Description: Corresponds to amino acid residues A302 to L309. The effect of Y306 phosphorylation is unclear. This is a major in vivo phosphorylation site in PPP1CB based on ≥314 mass spectrometry reports recorded in PhosphoSitePlus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.8 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Zebra fish | Honey bee | Sea Urchin.
Related Product 1: PPM1B Y367 phosphorylation antibody (Cat. No.: AB-PP540)
Related Product 2: PPP1CA Y134+Y137 phosphorylation antibody (Cat. No.: AB-PP541)
Related Product 3: PPP1CA Y306 phosphorylation antibody (Cat. No.: AB-PP542)
Related Product 4: PPP1CB T66 phosphorylation antibody (Cat. No.: AB-PP502)
Related Product 5: PPP2CA Y284 phosphorylation antibody (Cat. No.: AB-PP549)
Related Product 6: PPP2CA T646 phosphorylation antibody (Cat. No.: AB-PP504)
Related Product 7: PPP2CB T304 phosphorylation antibody (Cat. No.: AB-PP505)
Related Product 8: PPPM1A Y119 phosphorylation antibody (Cat. No.: AB-PP508)
Scientific Background: PPP1CB (Protein Phosphatase 1 Catalytic Subunit Beta) is a protein-serine/threonine phosphatase that belongs to the PPP phosphatase family, PP-1 subfamily. PPP1CB is sometimes referred to as PP1-delta (PP1\(\delta \)) and is one of three catalytic subunits (alpha, beta and gamma) of PP1. As a broad specificity phosphatase, it regulates diverse processes like cell division, metabolism including glycogen synthesis and breakdown, muscle contraction, transcription and synaptic plasticity. It exists as a ~37 kDa catalytic subunit, widely expressed in tissues, and forms specific complexes with >200 regulatory proteins to control hundreds of targets. It is a component of the PTW/PP1 phosphatase complex, which functions in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. It regulates NEK2 function in terms of its kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. It is a regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In concert with CSNK1D and CSNK1E, it determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. It can directly dephosphorylate CSNK1D and CSNK1E. It dephosphorylates the 'Ser-418' residue of FOXP3 and inactivates it in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby rendering Treg cells functionally defective. Mutations in the PPP1CB gene are linked to Noonan syndrome-like disorder with loose anagen hair 2, a condition often treated with growth hormone. This description may include information annotated by UniProt and/or Google AI.