Product Name:
PHLPP1-pY1712+pY1713
Product Number:
ab-pp529
Target Full Name: PH domain leucine-rich repeat-containing protein phosphatase 1
Target Alias: KIAA0606; pleckstrin homology domain containing family E protein; PLEKHEI, SCOP
Product Type Specific: PHLPP1 protein phosphatase phosphosite-specific antibody
Antibody Code: PP529
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1712+Y1713
Protein UniProt: O60346
Protein SigNET: PHLPP1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PHLPP1
Antibody Immunogen Sequence: LPD(pY)(pY)DTP(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues L1709 to P1716. The effects of Y1712 and Y1713 phosphorylation are unclear. These are major sites of in vivo phosphorylation by mass spectrometry (≥10 and ≥5 reports, respectively, in PhosphoSitePlus).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Rat | Mouse.
Related Product 1: PHLPP2 expression antibody (Cat. No.: AB-NP050-1)
Scientific Background: PHLPP1 (PH domain leucine-rich repeat-containing protein phosphatase 1) is a protein phosphatase involved in regulation of Akt and PKC signaling. It mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family. It specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA. Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumour growth. PHLPP1 features a Pleckstrin homology (PH) domain for membrane localization, leucine-rich repeats (LRR) for substrate recognition, a phosphatase domain (PP2C), and a PDZ binding motif. It exists as two main isoforms, PHLPP1-alpha) (133 kDa) and PHLPP1-beta) (185 kDa). Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. It dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. It dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. It dephosphorylates RAF1 inhibiting its kinase activity. It may act as a negative regulator of K-Ras signaling in membrane rafts. It is involved in the hippocampus-dependent long-term memory formation, in circadian control by regulating the consolidation of circadian periodicity after resetting, and in development and function of regulatory T-cells. In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue, but is largely excluded from the crypt bases. Overexpression inhibits cancer growth, while deficiency can lead to increased tumour growth. Elevated PHLPP1 levels have been linked to insulin resistance and diabetes by interfering with Akt-mediated signaling. It inhibits cancer cell proliferation and may act as a tumour suppressor. Its expression is lost or significantly decreased in 78% of tested tumours (at protein level). It is ubiquitously expressed in non-cancerous tissues. This description may include information annotated by UniProt and/or Google AI.