Product Name:
PTPRM-pY929
Product Number:
ab-pp526
Target Full Name: Receptor-type tyrosine-protein phosphatase mu
Target Alias: EC 3.1.3.48; HR-PTPu; Protein-tyrosine phosphatase mu; PTPRL1; RPTPM; R-PTP-MU; RPTPU
Product Type Specific: PTPRM protein phosphatase phosphosite-specific antibody
Antibody Code: PP526
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y929
Protein UniProt: P28827
Protein SigNET: PTPRM
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PTPRM
Antibody Immunogen Sequence: KNR(pY)GNI(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues K926 to I932. The effect of Y929 phosphorylation is unclear. This is the major site of in vivo phosphorylation of PTPRM (≥75 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in vertebrates and also found in the nematode worm.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rat | Mouse | Fruit fly | Nematode worm.
Related Product 1: PTPRA Y798 phosphorylation antibody (Cat. No.: AB-PP521)
Related Product 2: PTPRB Y1981 phosphorylation antibody (Cat. No.: AB-PP522)
Related Product 3: PTPRE Y696 phosphorylation antibody (Cat. No.: AB-PP554)
Related Product 4: PTPRF Y1621 phosphorylation antibody (Cat. No.: AB-PP523)
Related Product 5: PTPRK Y858 phosphorylation antibody (Cat. No.: AB-PP539)
Related Product 6: PTPRK Y916 phosphorylation antibody (Cat. No.: AB-PP524)
Related Product 7: PTRF Y308 phosphorylation antibody (Cat. No.: AB-PN533)
Related Product 8: PTRF Y308 phosphorylation antibody (Cat. No.: AB-PN646)
Scientific Background: PTPRM (Protein Tyrosine Phosphatase Receptor Type M; RPTP-mu) is a transmembrane protein phosphatase that belongs to the protein-tyrosine phosphatase family, Receptor class 2B subfamily. It regulates cell-cell adhesion, motility, and growth by dephosphorylating tyrosine residues on target proteins. It functions in controlling cell-cell interactions, promoting adipogenic differentiation, regulating neuronal axon guidance, and maintaining tissue architecture. PTPRM's structural design allows it to act as a "distance gauge" between cells while simultaneously transducing signals. PTPRM has an extracellular region, a single transmembrane region, and two tandem intracellular catalytic domains. It acts as a homophilic adhesion molecule, binding to PTPRM molecules on adjacent cells. It regulates phosphorylation of p120 catenin (CTNND1), epidermal growth factor receptor (EGFR) signaling, and impacts cadherin-dependent cell-cell adhesion. It is found primarily in the brain and epithelial tissues. It is frequently involved in tumour suppression and neuronal development. Its down-regulation via promoter hypermethylation is observed in cancers such as colorectal cancer, gliomas, and breast cancer. This description may include information annotated by UniProt and/or Google AI.