Product Name:
PTPRK-pY916
Product Number:
ab-pp524
Target Full Name: Receptor-type tyrosine-protein phosphatase kappa
Target Alias: EC 3.1.3.48; Protein tyrosine phosphatase, receptor type, K; Protein-tyrosine phosphatase kappa; PTPK; PTP-kappa; R-PTP-kappa
Product Type Specific: PTPRK protein phosphatase phosphosite-specific antibody
Antibody Code: PP524
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y916
Protein UniProt: Q15262
Protein SigNET: PTPRK
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PTPRK
Antibody Immunogen Sequence: CAKNR(pY)GNI
Antibody Immunogen Description: Corresponds to amino acid residues A912 to I919. The effect of Y916 phosphorylation is unclear. This is one of the major sites of in vivo phosphorylation of PTPRK (≥75 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in vertebrates and also found in the nematode worm.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Fruit fly | Nematode worm.
Related Product 1: PTPRA Y798 phosphorylation antibody (Cat. No.: AB-PP521)
Related Product 2: PTPRB Y1981 phosphorylation antibody (Cat. No.: AB-PP522)
Related Product 3: PTPRE Y696 phosphorylation antibody (Cat. No.: AB-PP554)
Related Product 4: PTPRF Y1621 phosphorylation antibody (Cat. No.: AB-PP523)
Related Product 5: PTPRK Y858 phosphorylation antibody (Cat. No.: AB-PP539)
Related Product 6: PTPRM Y929 phosphorylation antibody (Cat. No.: AB-PP526)
Related Product 7: PTRF Y308 phosphorylation antibody (Cat. No.: AB-PN533)
Related Product 8: PTRF Y308 phosphorylation antibody (Cat. No.: AB-PN646)
Scientific Background: PTPRK (Protein Tyrosine Phosphatase Receptor Type K; PTP-kappa) is a transmembrane phosphatase that belongs to the protein-tyrosine phosphatase family, Receptor class 2B subfamily. It regulates cell-cell adhesion, growth, and differentiation. It is a negative regulator of EGFR signaling pathway. It forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTPRK. It features an extracellular domain, a transmembrane region, and two tandem intracellular catalytic domains. The extracellular portion includes a MAM domain, an Ig-like domain, and four fibronectin type III-like repeats. It is highly expressed in lung, brain and colon, and to a lesser degree in liver, pancreas, stomach, kidney, and placenta. It acts as a tumour suppressor in cancers like colorectal and breast. Downregulation of PTPRK is associated with poor prognosis and increased cancer metastasis. This description may include information annotated by UniProt and/or Google AI.