Product Name:
PTPRF-pY1621
Product Number:
ab-pp523
Target Full Name: Receptor-type tyrosine-protein phosphatase F; Leukocyte antigen-related (LAR) PTP receptor
Target Alias: EC 3.1.3.48; LAR; LCA-homolog; Leukocyte antigen related; Leukocyte antigen-related (LAR) PTP receptor; Leukocyte antigen-related tyrosine phosphatase; Protein tyrosine phosphatase, receptor type, F; Receptor-type tyrosine-protein phosphatase F
Product Type Specific: PTPRF protein phosphatase phosphosite-specific antibody
Antibody Code: PP523
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1621
Protein UniProt: P10586
Protein SigNET: PTPRF
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PTPRF
Antibody Immunogen Sequence: CARNL(pY)AHI
Antibody Immunogen Description: Corresponds to amino acid residues A1617 to I1624. The effect of Y1621 phosphorylation is unclear. This is the major site of in vivo phosphorylation of PTPRF (≥143 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in vertebrates and also found in fish.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.6 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Frog | Zebra fish.
Related Product 1: PTPRA Y798 phosphorylation antibody (Cat. No.: AB-PP521)
Related Product 2: PTPRB Y1981 phosphorylation antibody (Cat. No.: AB-PP522)
Related Product 3: PTPRE Y696 phosphorylation antibody (Cat. No.: AB-PP554)
Related Product 4: PTPRK Y858 phosphorylation antibody (Cat. No.: AB-PP539)
Related Product 5: PTPRK Y916 phosphorylation antibody (Cat. No.: AB-PP524)
Related Product 6: PTPRM Y929 phosphorylation antibody (Cat. No.: AB-PP526)
Related Product 7: PTRF Y308 phosphorylation antibody (Cat. No.: AB-PN533)
Related Product 8: PTRF Y308 phosphorylation antibody (Cat. No.: AB-PN646)
Scientific Background: PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule. PTPRF removes phosphate group from β-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function (1,2,3,4). Anti-PTPRF (C-terminal) antibody is specific for the extracellular and cytoplasmic subunits of human PTPRF (approx. 210, 150 and 85 kDa). Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide. This description may include information annotated by UniProt and/or Google AI.