Product Name:
PTPRB-pY1981
Product Number:
ab-pp522
Target Full Name: Receptor-type tyrosine-protein phosphatase beta
Target Alias: EC 3.1.3.48; Protein tyrosine phosphatase, receptor type, B; PTPB; PTP-beta; R-PTP-beta; Vascular endothelial protein tyrosine phosphatase
Product Type Specific: PTPRB protein phosphatase phosphosite-specific antibody
Antibody Code: PP522
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y1981
Protein UniProt: P23467
Protein SigNET: PTPRB
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PTPRB
Antibody Immunogen Sequence: FPI(pY)ENV(βA)C
Antibody Immunogen Description: Corresponds to amino acid residues F1978 to V1984. The effect of Y1981 phosphorylation is unclear. This is the major site of in vivo phosphorylation of PTPRB (≥39 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is highly conserved in mammals and also found in the chicken.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus.
Related Product 1: PTPRA Y798 phosphorylation antibody (Cat. No.: AB-PP521)
Related Product 2: PTPRE Y696 phosphorylation antibody (Cat. No.: AB-PP554)
Related Product 3: PTPRF Y1621 phosphorylation antibody (Cat. No.: AB-PP523)
Related Product 4: PTPRK Y858 phosphorylation antibody (Cat. No.: AB-PP539)
Related Product 5: PTPRK Y916 phosphorylation antibody (Cat. No.: AB-PP524)
Related Product 6: PTPRM Y929 phosphorylation antibody (Cat. No.: AB-PP526)
Related Product 7: PTRF Y308 phosphorylation antibody (Cat. No.: AB-PN533)
Related Product 8: PTRF Y308 phosphorylation antibody (Cat. No.: AB-PN646)
Scientific Background: PTPRB (Protein Tyrosine Phosphatase Receptor Type B), also known as VE-PTP, is a transmembrane enzyme that belongs to the protein-tyrosine phosphatase family, Receptor class 3 subfamily. It is primarily expressed in endothelial cells, acting as an important regulator of vascular development, angiogenesis, and vascular integrity. As a negative regulator of receptor tyrosine kinase (RTK) signaling, it controls angiogenesis (blood vessel formation) and vascular stability. It specifically dephosphorylates key receptor tyrosine kinases (e.g., Tie2, VEGFR2) and VE-cadherin (CDH5) in endothelial cells, which directly impacts endothelial cell-cell adhesion and tube formation. It negatively controls TIE2 driven endothelial cell proliferation, which in turn affects blood vessel remodeling during embryonic development and determines blood vessel size during perinatal growth. It is essential for the maintenance of endothelial cell contact integrity and for the adhesive function of VE-cadherin in endothelial cells and this requires the presence of plakoglobin. It features an extensive extracellular domain with multiple fibronectin type III repeats, a single transmembrane segment, and one intracytoplasmic catalytic phosphatase domain. It is inhibited by zinc(II) ions and can be inactivated by dimerization, a process that can be triggered by binding to specific molecules. Mutations in the PTPRB gene are associated with abnormal vascular development and malignant vascular tumours such as angiosarcomas. It also plays a role in mammary epithelial branching morphogenesis. This description may include information annotated by UniProt and/or Google AI.