Product Name:
PTPRA-pY798
Product Number:
ab-pp521
Target Full Name: Receptor-type tyrosine-protein phosphatase alpha
Target Alias: EC 3.1.3.48; HLPR; HPTPA; LCA-related phosphatase; LRP; Protein tyrosine phosphatase, receptor type, A; Protein-tyrosine phosphatase alpha; PTPA; PTP-alpha; PTPRL2; RPTPA; RPTPalpha; R-PTP-alpha
Product Type Specific: PTPRA protein phosphatase phosphosite-specific antibody
Antibody Code: PP521
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y798
Protein UniProt: P18433
Protein SigNET: PTPRA
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PTPRA
Antibody Immunogen Sequence: CAFSD(pY)ANF
Antibody Immunogen Description: Corresponds to amino acid residues A794 to F801. Y798 phosphorylation stimulates phosphatase activity and regulates cytoskeletal reorganization. This is a major site of in vivo phosphorylation of PTPRA (≥2407 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). PTPRA is known to be phosphorylated at this site in vitro by Src.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.8 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Frog.
Related Product 1: PTPRB Y1981 phosphorylation antibody (Cat. No.: AB-PP522)
Related Product 2: PTPRE Y696 phosphorylation antibody (Cat. No.: AB-PP554)
Related Product 3: PTPRF Y1621 phosphorylation antibody (Cat. No.: AB-PP523)
Related Product 4: PTPRK Y858 phosphorylation antibody (Cat. No.: AB-PP539)
Related Product 5: PTPRK Y916 phosphorylation antibody (Cat. No.: AB-PP524)
Related Product 6: PTPRM Y929 phosphorylation antibody (Cat. No.: AB-PP526)
Related Product 7: PTRF Y308 phosphorylation antibody (Cat. No.: AB-PN533)
Related Product 8: PTRF Y308 phosphorylation antibody (Cat. No.: AB-PN646)
Scientific Background: PTPRA (Protein Tyrosine Phosphatase Receptor Type A) belongs to the protein-tyrosine phosphatase family, Receptor class 4 subfamily. It regulates cell growth, differentiation, and adhesion by removing phosphate groups from tyrosine residues on target proteins. As a receptor-type PTP, it is ubiquitously expressed, crucial for activating Src family kinases, and implicated in progression and neuronal migration. Its functions include dephosphorylating and activating Src family kinases, regulating integrin-mediated signaling, focal adhesion assembly, and modulating synaptic transmission. It features an extracellular domain, a single transmembrane segment, and two tandem intracytoplasmic catalytic domains. It is found in the plasma membrane and focal adhesions. Elevated expression is linked to tumour progression in various cancers, including breast, colon, and lung cancer. It acts as a regulator in RET signaling for medullary thyroid cancer and is involved in microbial pathogenesis, such as the action of Helicobacter pylori VacA toxin. This description may include information annotated by UniProt and/or Google AI.