Product Name:
PTPN3-pS357+pS359
Product Number:
ab-pp519
Target Full Name: Tyrosine-protein phosphatase non-receptor type 3
Target Alias: EC 3.1.3.48; Protein tyrosine phosphatase, non-receptor type 3; Protein-tyrosine phosphatase H1; PTN3; PTPH1; PTP-H1
Product Type Specific: PTPN3 protein phosphatase phosphosite-specific antibody
Antibody Code: PP519
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: S357+S359
Protein UniProt: P26045
Protein SigNET: PTPN3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PTPN3
Antibody Immunogen Sequence: CAMRR(pS)L(pS)VEH
Antibody Immunogen Description: Corresponds to amino acid residues A353 to H362. S359 phosphorylation induces interaction with 14-3-3. This is a major site of in vivo phosphorylation of PTPN3 (≥26 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). PTPN3 is known to be phosphorylated in vitro at S359 by TAK1 (MAP3K7).
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken.
Related Product 1: PTP1B Y20 phosphorylation antibody (Cat. No.: AB-PP532)
Related Product 2: PTP1B Y46 phosphorylation antibody (Cat. No.: AB-PP533)
Related Product 3: PTP1B S50 phosphorylation antibody (Cat. No.: AB-PP510)
Related Product 4: PTP1B Y362 phosphorylation antibody (Cat. No.: AB-PP509)
Related Product 5: PTPN2 S486 phosphorylation antibody (Cat. No.: AB-PP515)
Scientific Background: PTPN3 (Protein tyrosine phosphatase non-receptor type 3; PTPH) belongs to the protein-tyrosine phosphatase family, Non-receptor class subfamily. It regulates cell growth, differentiation, and migration by dephosphorylating tyrosine residues. It features an N-terminal FERM domain (subcellular localization), a central PDZ domain (protein-protein interactions), and a C-terminal PTP catalytic domain. It may act at junctions between the membrane and the cytoskeleton. It functions as a molecular switch, often acting as a tumour suppressor in cancers like renal cell carcinoma but promoting tumour progression in others. It involved in regulating MAPK, PI3K/AKT, and TGF-β signaling pathways. It acts as a phosphatase for substrates like p38γ MAPK, affecting cell proliferation, migration, and invasion. It is primarily located in the cytoplasm and associated with the cytoplasmic side of the plasma membrane. PTPN3 displays dual roles, behaving as a tumour suppressor in certain cancers (e.g., inhibiting proliferation in clear cell renal cell carcinoma) while acting as an oncogene in others (e.g., driving progression in pancreatic and cholangiocarcinoma). This description may include information annotated by UniProt and/or Google AI.