Product Name:
PTPN22-pY499
Product Number:
ab-pp517
Target Full Name: Protein-tyrosine phosphatase, non-receptor type 22
Target Alias: EC 3.1.3.48; Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP; Lymphoid phosphatase; LyP; Lyp1; Lyp2; PEP; PTN22; PTPN8
Product Type Specific: PTPN22 protein phosphatase phosphosite-specific antibody
Antibody Code: PP517
Antibody Target Type: Phosphosite-specific
Antibody Phosphosite: Y499
Protein UniProt: Q9Y2R2
Protein SigNET: PTPN22
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic phosphopeptide patterned after human PTPN22
Antibody Immunogen Sequence: CAELN(pY)SLP
Antibody Immunogen Description: Corresponds to amino acid residues A495 to P502. The effect of Y499 phosphorylation is unclear. This is the major site of in vivo phosphorylation of PTPN22 (≥59 reports from high throughput mass spectrometry studies recorded in PhosphoSitePlus). This human phosphosite is conserved in mammals and found in rat.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target phosphoprotein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse.
Related Product 1: PTPN21 S304 phosphorylation antibody (Cat. No.: AB-PP516)
Related Product 2: PTPN23 Y499 phosphorylation antibody (Cat. No.: AB-PP518)
Related Product 3: PTPN23 Y1229 phosphorylation antibody (Cat. No.: AB-PP537)
Scientific Background: PTPN22 (Protein Tyrosine Phosphatase Non-Receptor Type 22; Lymphoid Phosphatase; LYP) is a hematopoietic-specific cytoplasmic, 92 kDa enzyme that belongs to the protein-tyrosine phosphatase family, Non-receptor class 4 subfamily. It acts as a powerful negative regulator of immune cell signaling to prevent autoimmunity, particularly targeting T-cell receptor (TCR) and B-cell receptor (BCR) pathways, and maintains immune homeostasis and sets the threshold for activation. It functions as negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK (activating Tyr-394 site) and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70 (activating Tyr-493 site), VAV, VCP, SKAP2 and other key signaling molecules. It associates with and probably dephosphorylates CBL. It positively regulates toll-like receptor (TLR)-induced type 1 interferon production, and promotes host antiviral responses mediated by type 1 interferon. It regulates NOD2-induced pro-inflammatory cytokine secretion and autophagy. It is expressed in bone marrow, B and T-cells, PBMCs, natural killer cells, monocytes, dendritic cells and neutrophils. Both isoform 1 and 4 are predominantly expressed in lymphoid tissues and cells. Isoform 1 is expressed in thymocytes and both mature B and T-cells. A common polymorphism, R620W (or 1858C>T), is strongly linked to increased risk for multiple autoimmune diseases, including type 1 diabetes, rheumatoid arthritis, and SLE. The R620W variant is believed to enhance the phosphatase activity of LYP, leading to stronger inhibition of T-cell activation. This description may include information annotated by UniProt and/or Google AI.